Establishment of determination method of cherry blossom fragrance based on electronic nose technology
[Objective]In order to establish a rapid detection method of cherry blossom fragrance based on electronic nose technology and apply it to cherry blossom germplasm identification.[Method]Cerasus serrulate was used as the test material,and orthogonal design was used to analyze the effects of different headspace balance time,sample size,flowering period and sampling time on the determination of cherry blossom fragrance by electronic nose,and to screen the best determination parameters.Three cherry germplasm,namely,C.serrulate,C.yedoensis and C.serrulata var.lannesiana,were used to verify the effect.[Result]Sensors W1C,W5S,W1S,W1W,W2W and W3S had larger response values to cherry blossom aroma components,while other sensors hadsmaller response values(G/G0≈1).The response value of electronic nose sensor was significantly different under different factors,and the order of influence of each factor on the response of electronic nose sensor was flowering period＞sample size＞sampling time＞headspace balance time.According to the high response value,it was determined that the best combination of electronic nose for measuring the fragrance of cherry blossoms was A2B4C3D2,that was,4 g of flowers in full bloom were collected at 12:00,and measured after 30 min of headspace balance.Based on the best electronic nose measurement parameters,combined with principal component analysis(PCA)and linear judgment analysis(LDA),different cherry germplasm were distinguished.It was found that PCA and LDA analysis could distinguish different cherry germplasm well,and the discrimination degree of LDA method was higher than that of PCA method(LDA method discrimination degree=1;The discrimination degree of PCA method was 0.677-0.974),and the discrimination effect of LDA method was better than PCA method.The load factor of Loading analysis showed that sensors W1W,W2W,W5S,W1S,W2S and W3S had positive contributions to first principal component,and W6S had a main positive contribution to the second principal component.It showed that alkanes,nitrogen oxides and sulfides might be the main components to distinguish the fragrance types of cherry germplasm.[Conclusion]The best parameters for measuring the fragrance of cherry blossoms by electronic nose are as follows:4 g of blooming flowers are collected at 12:00,and measured after 30 min of headspace balance,and different germplasm are distinguished by LDA method,with high discrimination(discrimination=1).
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