Genetic diversity and genetic relationship analysis of Arenga pinnata germplasm resources based on ISSR markers
[Objective]In order to analysis the genetic diversity level of Guangxi Arenga pinnata,clarify the genetic relationship between the different A.pinnata,which provided a reference for the development utilization and protection of A.pinnata germplasm resources.[Method]The healthy,fresh and well-performing tender leaves were selected,and the genomic DNAs of 201 samples from 18 A.pinnata populations in Guangxi province were extracted using DNA Kit.Ten clearly amplified and highly polymorphic ISSR primers were used to PCR polymorphism amplification the genomic DNAs of A.pinnata samples by ISSR molecular marker technology,the percentage of polymorphic loci,the indexes of Nei's genetic diversity and Shannon information were estimated by POPGENE32,a cluster analysis graph among cultivars was built using and NTSY-pc software,discuss and analysis the genetic diversity and relationship of germplasm resources.Based on the resource status of A.pinnata genetic diversity and the genetic structure of corresponding populations,conservation strategies for A.pinnata germplasm resources were discussed.[Result]Ten primers amplified a total of 150 sites,including 150 polymorphic sites,and the polymorphism site ratio was 100%,the average number of observed alleles was 1.552 2,with the average number of effective alleles of 1.278 6,the average value of Shannon information index(I)was 0.257 8,the average Nei's gene diversity index(He)was 0.168 1.The Nonggang population had the highest genetic diversity(He=0.214 4),and the People's Park population had the lowest genetic diversity(He=0.104 7).Cluster analysis by UPGMA method showed that the genetic similarity coefficient of the 201 test materials ranged from 0.833 8 to 0.983 2,with an average of 0.9162,among that,Tiandeng county Chongzuo and the People's Park populations had the smallest genetic similarity coefficient,the genetic difference was the greatest and the kinship was the distantest;The coefficient of genetic similarity between Longzhou county Shuikou town and Wude town populations was the highest,the genetic difference was the smallest,and the genetic relationship was the closest.Based on UPGMA clustering analysis,the 18 populations could be divided into 5 groups while the genetic similarity coefficient was 0.928.[Conclusion]The genetic diversity level of A.pinnata is relatively high,and ISSR molecular markers are rich polymorphism in A.pinnata germplasms,which can reveal the relationship between A.pinnata populations,can be used to study the genetic diversity of A.pinnata.
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