Effect of plant growth regulators on the photosynthetic accumulation and flavonoid synthesis of Cylocarya paliurus
[Objective]The objective of this study is to provide a reference for the promotion of dwarf economic forests in Cylocarya paliurus.[Method]The 1-year-old seedlings were selected as the research object,and three growth regulators,four mass concentration levels,which were chlormequat chloride(500,1 000,1 500,2 000 mg/L),triiodobenzoic acid(50,100,150,200 mg/L),brassinolide(0.05,0.10,0.15,0.20 mg/L),were set respectively,the foliar fertilizer was sprayed on the plant leaves.Using clean water treatment as a control(CK),and the changes of photosynthetic accumulation and flavonoid synthesis related indexes of C.paliurus seedlings under different plant growth regulator treatments were explored.The principal component analysis was used to screen the optimal combination of species and concentration suitable for the cultivation of C.paliurus seedlings.[Result]Three plant growth regulators could increase the relative chlorophyll content of leaves of C.paliurus seedlings,and the 500 mg/L chlormequat chloride treatment had the most significant effect,which was 35.5%higher than that of CK treatment.However,the application of chlormequat chloride inhibited the synthesis of soluble sugar,soluble starch and non-structural carbohydrates in the leaves of C.paliurus seedlings,while the effect of triiodobenzoic acid treatment was the opposite,and the content of soluble sugars and non-structural carbohydrates in leaves reached the maximum in 50 mg/L triiodobenzoic acid treatment,which was 71.6%and 29.1%higher than that of CK,respectively.The soluble starch content of leaves reached the maximum in 150 mg/L triiodobenzoic acid treatment,which was 21.7%higher than that of CK treatment.The application of plant growth regulators would affect the ratio of photosynthetic to non-photosynthetic tissue biomass of C.paliurus seedlings,and the intermediate concentration of growth regulators was more conducive to the increase of the ratio of photosynthetic to non-photosynthetic tissue biomass.In terms of flavonoid synthesis,all three plant growth regulators enhanced the activity of two key enzymes,namely phenylalanine ammonialyase and chalcone synthase,involved in flavonoid synthesis in C.paliurus seedlings to a certain extent,and the total flavonoid content in the leaves of 100 mg/L triiodobenzoic acid treatmentwas the highest,which was 34.9%higher than that of CK treatment.The enzyme activities of PAL and CHS reached their maximum in 1500 mg/L chlormequatin treatment and 0.2 mg/L brassinolide treatment,respectively.[Conclusion]Chlormequat chloride,triiodobenzoic acid,and brassinolide all have certain regulatory effects on the photosynthetic accumulation and flavonoid synthesis of C.paliurus seedlings,and most of them have a promoting effect,150 mg/L triiodobenzoic acid has the best promoting effect,followed by 500 mg/L chlormequat chloride and 0.05 mg/L brassinolide.Overall,treatment with different concentrations of triiodobenzoic acid has a significant positive effect on the photosynthetic accumulation and flavonoid synthesis of C.paliurus,making it more suitable for dwarf cultivation of C.paliurus.
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