摘要
[目的]为沙棘酚酸和黄酮资源开发利用及定向育种提供参考.[方法]选取11个沙棘品种作为研究对象,采用液质联用(HPLC-MS/MS)方法检测各品种叶片和果实中酚酸类及黄酮类成分及其含量,并进行差异比较分析.[结果]在 11 个沙棘品种叶片中均可检测出阿魏酸、香草酸、对香豆酸等 10 种酚酸类成分,总含量为1 032.466～3 354.984 μg/g,其中'中红果'(3 354.984 μg/g)和'201309'(2 066.444 μg/g)叶片中总酚酸含量最高,沙棘叶片主要酚酸类成分为阿魏酸、香草酸、对香豆酸和没食子酸;在11 个沙棘品种叶片中均可检测出芦丁、表没食子儿茶素、槲皮素等 8 种黄酮类成分,总含量为 181.359～617.098 μg/g,以芦丁为主要成分,总黄酮含量最高的品种同样为'中红果'(617.098 μg/g)和'201309'(451.432 μg/g).在 11 个品种果实中可检测出原儿茶酸、丁香酸、对香豆酸、没食子酸、咖啡酸5种酚酸类成分,总含量为25.002～98.009 μg/g,'杂雌优10号'(98.009 μg/g)和'201308'(77.503 μg/g)果实中酚酸含量较高,对香豆酸为主要酚酸类成分;在 11 个品种果实中均可检测出 8 种黄酮类成分,总含量为 54.219～351.622 μg/g,'201301'(351.622 μg/g)和'201308'(189.981 μg/g)果实中含量较高,主要黄酮类成分同样为芦丁.[结论]'中红果'和'201309'可被作为沙棘叶片酚酸类和黄酮类种质资源,'杂雌优10 号''201308''201301'可被作为沙棘果实酚酸类和黄酮类种质资源进行定向培育和开发利用.
Abstract
[Objective]Provide reference for the development and utilization of seabuckthorn phenolic acid and flavonoid resources,as well as targeted breeding.[Method]Eleven sea buckthorn varieties were selected as the research objects,and liquid chromatography-mass spectrometry(HPLC-MS/MS)was used to detect the phenolic acid and flavonoid components in the leaves and berries of each variety,and differences were compared and analyzed.[Result]Ten phenolic acid components,including ferulic acid,vanillic acid,and p-coumaric acid,were detected in the leaves of 11 seabuckthorn varieties,with a total content of 1 032.466-3 354.984 μg/g.Among them,'Zhonghongguo'(3 354.984 μg/g)and'201309'(2066.444 μg/g)had the highest total phenolic acid content,mainly composed of ferulic acid,vanillic acid,p-coumaric acid,and gallic acid.Eight flavonoids,including rutin,epigallocatechin gallate,and quercetin,were detected in the leaves of 11 sea buckthorn varieties,with a total content of 181.359-617.098 μg/g.Rutin was the main component,and the varieties with the highest total flavonoid content were also'Zhonghongguo'(617.098 μg/g)and'201309'(451.432 μg/g).Five phenolic acids,namely protocatechuic acid,syringic acid,p-coumaric acid,gallic acid,and caffeic acid,were detected in the berries of 11 varieties,with a total content of 25.002-98.009 μg/g.The phenolic acid content of'Zaciyou 10'(98.009 μg/g)and'201308'(77.503 μg/g)was relatively high,with p-coumaric acid being the main component.Eight types of flavonoids could be detected in the berries of 11 varieties,with a total content of 54.219-351.622 μg/g.The content of'201301'(351.622 μg/g)and'201308'(189.981 μg/g)was relatively high,and the main flavonoid component was also rutin.[Conclusion]'Zhonghongguo'and'201309'can be used as phenolic acid and flavonoid resources in seabuckthorn leaves,while'Zaciyou10','201308'and'201301'can be used as phenolic acid and flavonoid resources in seabuckthorn berries for targeted cultivation and demonstration promotion.
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