Exploration of the mechanism of regulating uterus peristaltic wave by Shoutai Sijunzi Decoction based on network pharmacology
Objective To explore the potential target and mechanism of the regulation of uterus peristaltic wave by Shoutai Sijunzi Decoction based on network pharmacology and molecular docking techniques.Methods Based on Traditional Chi-nese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and Traditional Chinese Medicines Inte-grated Database(TCMID),the active ingredients and targets of Shoutai Sijunzi Decoction were screened.At the same time,Gencards and DrugBank databases were used to mine the relevant targets of uterus peristaltic waves,and the potential tar-gets of regulating abnormal endometrial peristalsis waves were obtained after the intersections were taken.The protein inter-action network of potential target was constructed using the STRING platform,and the"drug-active ingredients-intersection target-disease"network was constructed using Cytoscape software.The"clusterProfiler"package was used for gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.PyMOL and AuTo Dock software were used to verify the molecular docking between the core target and the active component monomer.Results A total of 594 targets of Shoutai Sijunzi Decoction were retrieved,90 targets of uterus peristaltic wave correlation,and 33 intersection targets were obtained.Kaempferol,β-sitosterol,quercetin,isorhamnetin and 7-methoxy-2-methyl isoflavones were the main active monomers of tetraphanoides,and nitric oxide synthase 3(NOS3),transient receptor potential vanilloid subtype(TRPV1),prostaglandin-endoperoxide synthase 2(PTGS2),caspase 3(CASP3)and prostaglandin-endoperoxide synthase 1(PT-GS1)were the core action targets.The KEGG pathway was mainly concentrated in neuroactive ligand-receptor interaction,calcium signaling pathway,5-hydroxytryptaminergic synapse,renin secretion,inflammatory mediators regulation of transient receptor potential(TRP)channels,cyclic guanosinc monophosphate-protein kinase G(cGMP-PKG)signaling pathway and relaxin signaling pathway.Molecular docking showed that kaempferol,β-sitosterol,quercetin,isorhamnetin and 7-methoxy-2-methyl isoflavone could stably bind to the core target.Conclusion Shoutai Sijunzi Decoction may target core genes such as NOS3,TRPV1,PTGS2,CASP3 and PTGS1 to regulate the neuroactive ligand-receptor interaction,calcium signaling pathway,5-hydroxytryptaminergic synapse,renin secretion,inflammatory mediators regulation of TRP channel,cGMP-PKG signaling pathway and relaxin signaling pathway.It plays the role of regulating the uterus peristaltic wave.
国家科技期刊平台
NSTL
万方数据
维普
