目的 探讨维生素B6(Vit B6)对肺动脉高压大鼠组织病理及DOK3/核因子-κB(NF-κB)信号通路的影响。方法 将30只雄性SD大鼠按随机数字表法分为实验组、模型组和对照组，每组10只，实验组、模型组给予野百合碱处理，实验组饲料中添加Vit B6，对照组给生理盐水，处理28 d。比较三组处理后大鼠血流动力学数据、组织病理和组织DOK3、NF-κB的表达水平。结果 模型组及实验组的右心室收缩压(RVSP)高于对照组，提示造模成功。实验组、模型组右心室肥厚指数、RVSP、管壁厚度(WT)、肌层面积与血管总面积比值(WA)均高于对照组，差异有统计学意义(P＜0。05);组织中DOK3、NF-κB的表达水平上升;给予Vit B6喂养后大鼠肺动脉高压指标显著改善，DOK3、NF-κB的表达下降，实验组与对照组的DOK3/NF-KB比较，差异无统计学意义(P＞0。05)。结论 Vit B6可通过DOK3/NF-κB信号通路改善肺动脉高压的血管重构。
Vitamin B6 regulates vascular remodeling in pulmonary hypertension through the DOK3/NF-κB signaling pathway
Objective To investigate the effect of vitamin B6(Vit B6)on histopathology and DOK3/nuclear factor-KB(NF-κB)signaling pathway in rats with pulmonary hypertension.Methods Thirty male SD rats were divided into experimental group,model group and control group according to random number table method,with 10 rats in each group.The experimental group and model group were treated with monocrotaline,the experimental group was added with Vit B6,and the control group was given normal saline for 28 days.The hemodynamic data,histopathology and the expression levels of DOK3 and NF-κB in the three groups were compared.Results The right ventricular systolic pressure(RVSP)in the model group and experimental group were higher than those in the control group,indicating successful modeling.Right ventricular hypertrophy index,RVSP,tube wall thickness(WT),ratio of muscle area to total vessel area(WA)in experimental group and model group were higher than those in control group,with statistical significance(P＜0.05).The expression levels of DOK3 and NF-κB in tissues were increased.After feeding with Vit B6,the pulmonary hypertension index of rats was significantly improved,and the expressions of DOK3 and NF-κB were decreased.There was no significant difference in DOK3/NF-κB between the experimental group and the control group(P＞0.05).Conclusion Vit B6 improves vascular remodeling in pulmonary hypertension via the DOK3/NF-κB signaling pathway.
万方数据
维普
