Inhibitory effect and mechanism of hepatocellular carcinoma cells upon treatment with the flavonoid compound GL-V9
Objective Hepatocellular carcinoma is one of the most common malignant tumors in the world,especially in China.The prognosis for patients with hepatocellular carcinoma is often poor due to the limited effectiveness of current treatments.The aim of this study was to investigate the inhibitory effect and mechanism of flavonoid GL-V9 on hepatocellu-lar carcinoma cells.Methods Cell counting kit-8(CCK-8 method)was used to detect the effects of different concentrations of GL-V9 on cell proliferation of hepatocellular cancer cell line Bel-7404 at different times.The effect of GL-V9 on the proliferation of Bel-7404 cells was determined by plate cloning.The effect of GL-V9 on apoptosis rate of Bel-7404 cells was analyzed by flow cytometry.The expression of autophagy markers LC3 and Beclin-1 in Bel-7404 cells were detected by Western blot assay with GL-V9.Results GL-V9 significantly inhibited the proliferation of hepatocellular carcinoma cell line Bel-7404,resulting in increased apoptosis rate of hepatocellular carcinoma cells,and significant changes in LC3 and Beclin-1 expression.Conclusion The significant inhibitory effect of GL-V9 on hepatocellular carcinoma cells may be relat-ed to the activation of autophagy pathway in hepatocellular carcinoma cell lines under the action of GL-V9.
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