Therapeutic effect and mechanism of Xixian Xiaobai Beverage on vitiligo model mice through regulating silent information regulator 1/peroxisome proliferator-activated receptor y coactivator-1α pathway
Objective To explore the effect of Xixian Xiaobai Beverage on skin and fur decolorization,inflammation,and expression of silent information regulator 1(SIRT1)/peroxisome proliferator-activated receptor γ coactivator-1 α(PGC-1 α)pathway proteins on vitiligo model mice and the underlying mechanism.Methods A total of 40 SPF only 6 weeks of health male C57BL/6 mice were randomly divided into control group,model group,positive drug group and Xixian Xiaobai Beverage group.Model group,positive drug group and Xixian Xiaobai Beverage group were established by embro-cating 5％hydrogen peroxide(H2O2)for 50 consecutive days.The Xixian Xiaobai Beverage group were given Xixian Xiaobai Beverage 147 mg/(kg·d)by gavage on the 21s day of modeling.The positive drug group were given Baidianfeng Capsules 464 mg/(kg·d)by gavage.The skin and hair decolorization were observed and recorded,Masson-Fontana staining was used to observe and calculate the percentage of melanin expression area and melanin distribution score of the epidermis.The rate of melanin-containing hair follicles was statistically calculated.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of serum tyrosinase(TYR),malondialdehyde(MDA),superoxide dismutase(SOD)and 8-hydroxydeoxyguanosine(8-OHDG).Western blot was used to detect the protein expression levels of SIRT1,PGC-1 αand manganese SOD(MnSOD)in skin tissues.Results The percentage of epidermal melanin expression area,epidermal melanin distribution score,melanin hair follicle rate,contents of TYR and SOD in serum,expressions of MnSOD,SIRT1 and PGC-1 α in model group induced by H2O2 solution were lower than those in control group,while the expressions of MDA and 8-OHdG were higher than those in control group,the differences were statistically significant(P＜0.05).The percentage of melanin expression area,epidermal melanin distribution score,melanin hair follicle rate,contents of TYR and SOD in serum,expressions of MnSOD,SIRT1 and PGC-1 α in skin tissue in Xixian Xiaobai Beverage group were higher than those in control group,while the expressions of MDA and 8-OHdG were lower than those in model group,with statistical significance(P＜0.05).There was no significant difference in MnSOD protein expression between Xixian Xiaobai Beverage group and model group(P＞0.05).Conclusion Enhancement of oxidative stress induced by H2O2 solution in vitiligo mice may be related to the change of SIRT1/PGC-1 α pathway.Xixian Xiaobai Beverage can enhance the antioxidant capacity of skin tissues and prevent the occurrence and development of vitiligo by regulating the SIRT1/PGC-1 α signaling pathway.
万方数据
维普
