Study on the effect of Gynostemma pentaphyllum on bladder cancer rats based on the theory of"renal main water"
Objective To observe the effect of Gynostemma pentaphyllum on N-methyl-n-nitrosourea(MNU)-induced bladder cancer(BC)in SD rats based on the theory of"renal main water".Methods A total of 106 SPF SD rats were randomly divided into blank group,model group,low-dose group,medium-dose group,high-dose group and positive control group.Model group,low-dose group,medium-dose group,high-dose group and positive control group were given MNU by bladder perfusion for modeling,and blank group and model group were given 0.5 ml bladder perfusion of normal saline.Low dose group(20 mg/kg),medium dose group(40 mg/kg)and high dose group(80 mg/kg)were injected with aesculus chinensis powder.The positive control group was injected with lyophilized BCG vaccine powder.The morphology of the bladder in each group was observed by histological and pathological sections,and the bladder organ index of each group was calculated and related physiological indexes were detected.To evaluate the effect of bladder infusion of aesculus on BC induced by MNU in SD rats.Results At the 16th week,the bladder organ index of the model group was higher than that of the blank group,and the difference was statistically significant(P＜0.05).The bladder organ indexes of low-dose group,medium-dose group,high-dose group and positive control group were lower than those of model group,and the differences were statistically significant(P＜0.05).Conclusion Gynostemma pentaphyllum has certain pharmacological activity against bladder cancer.The experimental data can provide a certain reference for the development of anti-bladder cancer drugs.
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