Objective To study the expression of cholesterol transport-related proteins in hippocampus and liver tissues of naturally aging mice and observe the effect of aging on cholesterol transport.Methods Ten clean grade male Kunming mice at l month old and 10 months old were selected,1 month old mice were included in the control group,and 10 months old mice were included in the elder group.After 7 days of feeding,the behavior and memory of the mice were detected by Y-shaped maze,and the expression changes of fatty acid synthase(FASN)protein in liver and apolipoprotein E(ApoE)pro-tein in hippocampus were detected by immunohistochemistry.The contents of total cholesterol,triacylglycerol,high density cholesterol and low density cholesterol were detected by ELISA.The expressions of ATP binding cassette transporter A1(ABCA1),low-density lipoprotein receptor(LDLR)protein in liver and ABCA1 protein and ApoE protein in hippocampus were detected by Western blot.Results The elder group showed less food intake,lazy movement and slow reaction.The total number of arm insertion and alternation in the elder group were less than those in the control group,and the differences were statistically significant(P＜0.05).The content of high density lipoprotein cholesterol in the elder group was lower than that in the control group,and triglyceride and low density lipoprotein cholesterol were higher than those in the control group,the differences were statistically significant(P＜0.05).The levels of FASN,LDLR and ABCA1 of liver in the elder group were lower than those in the control group,the expression of ApoE protein in hippocampal tissues of the elder group was higher than that of the control group,and the expression of ABCA1 protein was lower than that of the control group,and the differ-ences were statistically significant(P＜0.05).Conclusion The abnormal peripheral and central cholesterol metabolism of aged mice may be related to the abnormal expression of ABCA1 protein,ApoE protein and LDLR protein in liver and hip-pocampus.
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