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丰城市食源性沙门氏菌的血清型、耐药性及同源性分析

Serotype,antimicrobial resistance and phylogeny of foodborne Salmonella in Fengcheng City

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目的 探究丰城市食源性沙门氏菌的血清型分布特征、耐药性分析及脉冲场凝胶电泳(PFGE)分子溯源分析,为食源性疾病的暴发预警提供科学依据.方法 对丰城市2015年4月至2022年11月分离来自食品和腹泻患者的沙门氏菌进行血清学分型以及耐药性分析,应用PFGE分析菌株之间的同源关系.结果 在756份食品和806份腹泻患者样品中,共检出沙门氏菌49株,其中鼠伤寒沙门氏菌占比率为38.78%,其次肠炎沙门氏菌占比率为32.65%.选取的35株沙门氏菌对氨苄西林耐药率最高,耐药率达到82.86%,其次是对头孢唑啉和萘啶酸耐药率达到48.57%,其中30株具有多重耐药性(30/35,85.71%).49株沙门氏菌DNA指纹图谱同源性为55.4%~100.0%.结论 丰城市从食源性分离到的沙门氏菌株主要以鼠伤寒沙门氏菌和肠炎沙门氏菌为主,耐药和多重耐药存在严重,应加强对抗生素规范使用,DNA指纹图谱显示沙门氏菌较高同源性,需加强PFGE的日常监测为沙门氏菌的食品安全风险提供预警.
Objective Serotype distribution characteristics,antimicrobial resistance analysis and pulsed-field gel elec-trophoresis(PFGE)molecular traceability analysis were investigated in foodborne Salmonella from Fengcheng City,provid-ing scientific basis for early warning of foodborne disease outbreaks.Methods Salmonella was isolated from food and from patients with diarrhea in Fengxheng City from April 2015 to November 2022,serotype distribution characteristics and drug resistance analysis were investigated in Salmonella,and the evolutionary relationships between strains were analyzed by PFGE.Results A total of 49 strains of Salmonella were detected in 756 food samples and 806 diarrhea samples,the strains of which 38.78%were S.typhimurium and 32.65%were S.enteritidis.Thirty-five of these strains were showed antimicro-bial resistance,the highest resistance was against Ampicillin with 82.86%resistance,the next was against Cefazolin and Nalidixic Acid with 48.57%resistance.Thirty strains(30/35,85.71%)were found to be resistant to multiple antibiotics.Ho-mologies between 55.4%and 100.0%were observed among the 49 Salmonella strains.Conclusion S.typhimurium and S.enteritidis were isolated from foodborne in Fengcheng City as the main strains,drug resistance and multiple drug resistance are considered serious,drug resistance and multiple drug resistance are serious,the regulation of antibiotic should be strengthened.High homology of Salmonella was showed in DNA fingerprinting.The daily monitoring of PFGE should be strengthened to provide early warning for food safety risks of Salmonella.

SalmonellaSerotypeDrug resistancePulsed-field gel electrophoresis

邹锦群、詹卓蓬、彭思露、杨伟、彭洛霞、杨旋

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江西省丰城市疾病预防控制中心检验科,江西丰城 331100

江西省疾病预防控制中心营养与食品安全所,江西南昌 330046

沙门氏菌 血清型 耐药性 脉冲场凝胶电泳

江西省卫生健康委科技计划项目

202212599

2024

中国当代医药
中国保健协会 当代创新(北京)医药科学研究院

中国当代医药

影响因子:1.215
ISSN:1674-4721
年,卷(期):2024.31(6)
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