Study on the effect of extract of Radix isatidis on interleukin-6,tumor necrosis factor-α,interleukin-17A of Mycoplasma pneumoniae-infected mice in serum
Study on the effect of extract of Radix isatidis on interleukin-6,tumor necrosis factor-α,interleukin-17A of Mycoplasma pneumoniae-infected mice in serum
Objective To investigate the effects of Radix isatidis on serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interleukin-17A(IL-17A)in mice infected with Mycoplasma pneumoniae.Methods A total of 40 BALB/c mice were selected and randomly divided into control group,model group and 3 different concentrations group(250,125,62.5 mg/ml)of extract of Radix isatidis,including with 8 mice in each group.The mouse model of Mycoplasma pneumoniae infection was established by nasal drop method.After modeling success,mice were intragastrated with different concentra-tions of Radix isatidis extract,and the control group and the model group were intragastrated with the same amount of normal saline.The serum levels of IL-6,TNF-α and IL-17A in mice were detected by Cytometric.Hematoxylin-eosin staining was used to observe the pathological changes of lung tissue.Results Serum levels of IL-6,TNF-α and IL-17A in model group were higher than those in control group,the levels of IL-6 and IL-17A in Radix isatidis low concentration group,Radix isatidis medium concentration group and Radix isatidis high concentration group were lower than those in model group,and the level of TNF-α in Radix isatidis medium concentration group and Radix isatidis high concentration group was lower than those in model group,with statistical significances(P＜0.05).Conclusion Radix isatidis could alleviate the inflammatory le-sions in lung tissues caused by Mycoplasma pneumoniae infection and the release of excessive inflammatory factors,inhibit the excessive immune response,and alleviate the immune pathological damage.
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