Objective To investigate the effect of dioscorea porridge on insulin resistance and oxidative stress in rats with diabetes and stroke.Methods A total of 60 Wistar rats were selected,10 were used as blank group,and 45 rats were treated with abdominal injection of Streptozocin(STZ)and ZeaLonga thread thrombus.The rats with successfully constructed mem-branes were randomly divided into model group and dioscorea group,and 8 rats in blank group,12 rats in model group and 13 rats in dioscorea group.Model group and blank group were given 0.9％Sodium Chloride Injection 5 ml,and dioscorea group was given dioscorea porridge 5 ml,a total of 4 weeks.After the intervention,fasting blood glucose(FBG)was measured by portable glucose meter every 2 weeks,and oral glucose tolerance test(OGTT)was performed after the intervention for 4 weeks.Glycoylated hemoglobin(HBA1),fasting insulin(FINS)and homeostasis model assessment-insulin resistance(HOMA-IR)were detected by enzyme-related immunosorbent assay.The blood sugar of rats was detected,and the liver was taken to detect the oxidative stress markers superoxide dismutase(SOD)and malondialdehyde(MDA).The key proteins of insulin resistance signaling pathway(glucose transporter 4[GLUT-4],insulin receptor substrate 1[IRS-1],phosphorylated insulin receptor substrate 1[P-IRS-1]),and colon tissues of each group were taken 100 mg to detect the expression of glucagon-like peptide-1(GLP-1).Results Before the ex-periment,there were no statistical differences between the two groups(P＞0.05).After the experiment,FBG,HBA1,FINS and HOMA-IR of 2 weeks and 4 weeks rats in dios-genin group were lower than those in model group,and the differences were statistically significant(P＜0.05).The glucose tolerance of rats in diocorea group after OGTT was lower than that of model group,with statistical significance(P＜0.05).After the experiment,GLP-1 in dioscorea group was higher than that in model group,with statistical significance(P＜0.05).The key proteins of insulin resistance signaling pathway IRS-1,P-IRS-1 and GLUT-4 in dioscorea group were higher than those in model group,and the differences were statistically significant(P＜0.05).The oxidative stress markers MDA in dioscorea group was lower than those in model group,SOD was higher than that in model group,and the differences were statistically significant(P＜0.05).Conclusion Dioscorea porridge can effectively improve blood sugar,oxidative stress and IR in rats with diabetes and stroke,which is worthy of clinical application.
关键词
糖尿病脑卒中/薯蓣粥/氧化应激/胰岛素抵抗/实验研究
Key words
Diabetes and stroke/Dioscorea porridge/Oxidative stress/Insulin resistance/Experimental study
维普
万方数据