Analysis of the application effect of metabolomics markers to establish a diagnostic model for exercise fatigue
Objective To investigate the construction of diagnostic model of exercise fatigue with differential metabolites.Methods Ten male athletes were selected as the subjects of this study,and the Harvard step test was performed 30 steps/min for 5 min.Urine samples were collected before and after the test,their differential metabolites were detected by liquid chromatograph mass spectrometer(LC-MS),and the peak value of mass spectrum was preliminarily processed.Principal component analysis(PCA)and orthogonal partial least-squares discriminant analysis(OPLS-DA)were performed,and different metabolic pathways were connected through MetPA database.Results After test,the metabolites of capric acid,histidine,3-indoleacetic acid,corticosterone and creatine were lower than those before test,and pyroglutamate was higher than that before test,the differences were statistically significant(P＜0.05).The metabolic system associated with capric acid,histidine,3-indoleacetic acid,corticosterone,creatine,and pyroglutamate involves fatty acid biosynthesis,histidine metabolism,tryptophan metabolism,steroid hormone biosynthesis,arginine and proline metabolism,and glutathione metabolism.Conclusion Amino acid metabolism and steroid metabolism have obvious effects on athletes'body,and their related metabolites can be used as markers to evaluate sports fatigue,which is worthy of popularization and application.
AthletesHarvard step testDifferential metabolitesUrineExercise fatigueDiagnostic model
万方数据
维普
