Mechanism analysis of interleukin-33/ST2 signaling pathway regulating the immune balance of helper T cell/regulatory T cell in chronic obstruc-tive pulmonary disease
Mechanism analysis of interleukin-33/ST2 signaling pathway regulating the immune balance of helper T cell/regulatory T cell in chronic obstruc-tive pulmonary disease
Objective To explore the clinical mechanism of interleukin-33(IL-33)/ST2 signaling pathway activating the maturation of dendritic cells(DCs)and regulating the immune balance of helper T cell 17(Th17)/regulatory T cell(Treg)in mice with chronic obstructive pulmonary disease(COPD).Methods A total of 36 SPF C57BL/6 healthy mice were selected,12 mice were randomly selected as the healthy control group,and the remaining mice were used to establish the COPD model.The COPD model was established by cigarette smoke stimulation and aiiway injection of lipopolysaccharide.The model mice were divided into COPD group(n=12)and IL-33 antibody intervention group(n=12)according to the random number table method.The mice in IL-33 antibody group were intraperitoneally injected with IL-33 antibody at the third week after modeling,and the mice in healthy control group and COPD group were injected with the same dose of normal saline.On the 28th day after modeling,the blood samples from the inner canthus vein of the three groups of mice were collected while the mice were awake.The right lung tissue of the three groups of mice was washed with PBS solution and bronchoalveolar lavage fluid(BALF)was collected.The differences in the expression of DCs and IL-33 in peripheral blood and BALF of the three groups of mice were compared.Results The Th17/Treg ratio in peripheral blood and BALF of mice in COPD group were higher than those in healthy control group and IL-33 antibody intervention group,with statistically signifcant differences(P＜0.05).The levels of CD80 and CD86 in peripheral blood and BALF of mice in COPD group were higher than those in healthy control group and IL-33 an-tibody intervention group,and the major histocompatibility complex(MHC)-Ⅱ was lower than that in healthy control group and that in IL-33 antibody intervention group,with statistically signifcant differences(P＜0.05).RT-PCR analysis showed that the gray value of IL-33 in COPD group was higher than that in healthy control group and IL-33 antibody intervention group,with statistically signifcant differences(P＜0.05).The pathological differences of airway inflammation in the three groups of mice are great.Conclusion Regulating IL-33/ST2 signaling pathway can activate the maturation of DCs cells,re-store Th17/Treg balance in COPD mice,and improve the level of inflammatory indicators in COPD mice.
维普
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