Active ingredients and mechanism of Shenge Granules in treating non-al-coholic fatty liver were analyzed by ultra high liquid chromatography-four-pole time-of-flight tandem mass spectrometry and network phar-macology
Active ingredients and mechanism of Shenge Granules in treating non-al-coholic fatty liver were analyzed by ultra high liquid chromatography-four-pole time-of-flight tandem mass spectrometry and network phar-macology
Objective To explore the active ingredients and mechanism of Shenge Granules in the treatment of non-alcoholic fatty liver disease by using ultra high liquid chromatography-four-pole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)technique combined with network pharmacology.Methods The chemical components in Shenge Granules were identified using UPLC-Q-TOF-MS/MS.The active ingredients and targets of Shenge Granules were screened using the traditional Chinese medicine system pharmacology database(TCMSP),PubChem database,and SwissTargetPrediction database.Disease targets of non-alcoholic fatty liver disease were searched in databases such as GeneCards.A drug disease Venn diagram was drawn on the Venny 2.1.0 online plotting platform.The intersecting targets were imported into the String database and visualized using Cytoscape 3.10.0 software.At the same time,GO and KEGG pathway enrichment was performed using the DAVID database based on the intersecting targets.Construct a core target network diagram and a disease pathway target component drug network diagram using Cytoscape 3.10.0 software.Results A total of 75 compounds were analyzed by mass spectrometry,29 active ingredients,499 drug targets,4 776 disease targets,353 common targets and 10 key genes were obtained,respectively:TP53,CASP3,HIF1A,JUN,BCL2L1,SRC,MTOR,HRAS,TNF,ALB,and 8 key active ingredients,namely:salvianolic acid B,Puerolin,Puerol B,ononin,glucoside A,tanshensin,daidzein and genigeniin.Conclusion The treatment of non-alcoholic fatty liver with Shenge Granules has the characteristics of multi-component,multi-target and multi-pathway,and mainly plays a role in the treatment of non-alcoholic fatty liver disease by reducing liver fat accumulation,regulating endocrine,slowing inflammation and apoptosis.
Shenge Granules/Non-alcoholic fatty liver disease/Ultra high liquid chromatography-four-pole time-of-flight tandem mass spectrometry/Network pharmacology/Molecular docking/Mechanism of action
维普
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