Objective To investigate the effect and mechanism of Bushen-Qingre Prescription on alveolar bone resorption in rats with periodontitis.Methods A total of 54 SD rats were selected and divided into 6 groups(blank control group,model control group,positive control group and experimental groups[A1,A2,A3])according to random number table method,with 9 rats in each group.Except for the blank control group,the periodontitis model was induced by ligating the left maxillary first molar and drinking 10％sugar water in the other five groups.After the model was successfully established,the rats in the experimental groups(A1,A2,A3)were treated with Bushen-Qingre Prescription(3.5,7,14 ml/[kg·d])by gavage,re-spectively.Four weeks later,the left maxillary first molar and periodontal tissues were collected.The probing depth(PD)of the left upper first molar was measured,and the distance between cement to enamel junction(CEJ)and alveolar bone crest(ABC)of the left upper first molar was measured by Micro CT.And the protein expression of osteoprotegerin(OPG),recep-tor activator of NF-κB(RANK),receptor activator of NF-κB ligand(RANKL)were detected by Western Blot.Results After 4 weeks intervention,the PD of positive control group and experimental groups(A2,A3)were less than that in the model control group,and the differences were statistically significant(P＜0.05).There were no significant differences in PD between experimental group A1 and other groups(P＞0.05).Micro CT detection results showed that the CEJ-ABC distance of model control group was greater than that of the blank control group,and the difference was statistically significant(P＜0.05).The CEJ-ABC distance of the positive control group and the experimental groups(A1,A2,A3)were smaller than that of the model control group,and the differences were statistically significant(P＜0.05).Western Blot test results showed that the expression level of OPG in model control group and positive control group were lower than that in the blank control group,and the differences were statistically significant(P＜0.05).While there were no significant differences in OPG expression among other groups(P＞0.05).The expression levels of RANK and RANKL in the model control group were higher than those in the blank control group,the expression levels of RANK and RANKL in the positive control group and the experimental groups(A1,A2,A3)were lower than those in the model control group,and the expression levels of RANK and RANKL in the experimental groups(A2,A3)were lower than those in the positive control group,and the differences were statistically significant(P＜0.05).Conclusion Bushen-Qingre Prescription can inhibit alveolar bone absorption in periodontitis rats,and the potential mechanism may be involved in OPG/RANK/RANKL signaling system.
关键词
牙周炎/补肾清热方/骨保护素/核因子-κB受体活化因子/核因子-κB受体活化因子配体/牙槽骨吸收
Key words
Periodontitis/Bushen-Qingre Prescription/Osteoprotegerin/receptor activator of NF-KB/receptor activator of NF-κB ligand/Alveolar bone resorption
维普
万方数据