Mechanism of Yupingfeng Powder in the treatment of diabetic nephropa-thy based on network pharmacology and molecular docking
Objective To clarify the possible mechanism of Yupingfeng Powder in the adjuvant treatment of diabetic nephropathy by network pharmacology and molecular docking technology.Methods The Traditional Chinese Medicine Sys-tems Pharmacology Database and Analysis Platform(TCMSP)was used to search the chemical components and their action targets of Astragalus membranaceus,Paratractylodes and Atractylodes atractylodes in Yupingfeng Powder.The protein tar-gets were normalized to the corresponding gene names by Perl language.Through human genes related genes of diabetic nephropathy(GeneCards)database retrieval.After taking their intersection,the protein interaction network was constructed by STRING platform.The CytoScape software was used to build"pharmaceutical ingredients-targets"network.R language was used to perform gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,and the main components and core targets were screened for molecular docking study.Results There were 45 active components and 143 related target genes of Yupingfeng Powder in diabetic nephropathy.KEGG pathway enrichment analysis article 46 signaling pathways.Molecular docking results showed that quercetin,kaon-afol,isorhamnetin in Yupingfeng Powder had good binding activity with the main targets interleukin-6(IL-6),peroxisome proliferator-activated receptor gamma(PPARG)and vascular endothelial growth factor A(VEGFA).Conclusion This study preliminarly confirmed that Yupingfeng Powder can treat diabetic nephropathy through multi-components,multi-targets and multi-pathways,which provides a reference for further study of its active components and molecular mechanism.
万方数据
维普
