Objective To establish the high performance liquid chromatography(HPLC)fingerprint of Sanhuang Lotion,and analyze the correlation of medicinal materials and finished products,to provide a basis for the quality control of the prepara-tion.Methods The HPLC fingerprint analysis was performed on a 30℃ ZORBAX Eclipse XDB-C18 chromatographic col-umn(4.6 mm × 250 mm,5 μm),with the mobile phase consisting of methanol-0.1％phosphoric acid solution at the flow rate of 1.0 ml/min,the volume flow rate was 1.0 ml/min,the detection wavelength was set at 265 nm,and the injection vol-ume was 10 μl.Results The HPLC fingerprint of Sanhuang lotion was established,a total of 27 common peaks were cali-brated.Six components were identified as baicalin,scutellarin,baicalein,berberine,aloe-emodin and rhein,and the simi-larities of the 10 batches of preparations were all＞0.95.The correlation between medicinal materials and finished products was better.Conclusion The method is simple,accurate and reproducible,and can be used as a detection method for San-huang Lotion quality control.
维普
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