Objective To observe the changes in dendritic spines of primary motor cortex pyramidal neurons in Parkinson disease(PD)mouse model and explore possible mechanisms.Methods PD model was established using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP),and behavioral experiments and immunohistochemistry were used to identify the damage of dopamine-positive neurons in the substantia nigra.Golgi staining was used to observe the changes in dendrites and dendritic spines of pyramidal neurons.Immunohistochemical analysis revealed changes in serum induced kinases(SNK)and spine-associated Rap specific GTPase activation proteins(SPAR).Results The behavioral score,mean optical density of dopamine neurons and dendritic spine density in the MPTP group were lower than those in the normal control group,and the length of the dendritic spines in pyramidal neurons in the MPTP group was significantly higher than that in the normal con-trol group,and the differences were statistically significant(P＜0.05).There was no significant difference in the dendritic complexity of neurons between the two groups(P＞0.05).Conclusion The dendritic spines of cortical neurons in MPTP mice were damaged,and the changes in dendritic spines may be related to changes in SNK and SPAR expression.
维普
万方数据