Identification of Salvia miltiorrhiza from different place of origin and counterfeit based on near infrared reflectance spectroscopy
Objective To explore the effect of near infrared reflectance spectroscopy combined with chemometrics algorithm on the identification of Salvia miltiorrhiza and its counterfeits from different places.Methods A total of 265 batches of Salvia miltiorrhiza samples were collected from 9 producing areas in Shaanxi,Shandong,Sichuan and Hebei,and 61 batches of three common counterfeit Salvia miltiorrhiza products were collected.The near infrared diffuse reflection spectrum of the sample powder was collected by SabIR diffuse reflection fiber probe.Multiple scattering correction,standard canonical transformation,S-G smoothing,Norris smoothing,first-order differentiation and second-order differentiation were used to preprocess the spectra.TQ Analyst analysis system was applied,principal component analysis combined with Mahalanobis distance discriminant analysis method was used to determine the number of principal components,establish the origin iden-tification and authenticity identification models of Salvia miltiorrhiza,and four-fold cross-validation was used to test the prediction performance of the model.Results Using MSC+first-order+Norris spectral preprocessing method,the optimum principal component number was determined to be 10,the model was established and verified,and the accuracy of origin i-dentification was more than 98％,and the accuracy of authenticity identification was 100％.Conclusion This method can quickly and accurately identify the origin of Salvia miltiorrhiza and its counterfeit products,which is a scientific supplement to the existing methods.
Near infrared reflectance spectroscopySalvia miltiorrhizaPrinciple component analysisDiscriminative analysis
万方数据
维普
