Changes and clinical significance of plasma LncRNA NEAT1 and LMR levels in patients with acute coronary syndrome
Objective To investigate the correlation between plasma long non-coding RNA nuclear enriched abundant transcript 1(LncRNA NEAT1),lymphocyte to monocyte ratio(LMR)and acute coronary syndrome(ACS).Methods A total of 194 patients who were hospitalized in the Department of Cardiology,Loudi Central Hospital due to chest tightness and chest pain and underwent coronary angiography from January 2021 to January 2022 were selected as the research objects.Among them,50 cases with negative coronary angiography(coronary angiography showed normal coronary artery)were in-cluded in the control group.The remaining 144 patients with positive coronary angiography(ACS patients)were divided into unstable angina pectoris(UA)group(n=64)and acute myocardial infarction(AMI)group(n=80)according to the diagnostic criteria.The expression level of LncRNA NEAT1 in plasma was detected by real-time PCR.The lymphocyte count(LC)and monocyte count(MC)of blood routine were detected and collected,and the LMR level was calculated.Spearman correlation analysis was used to analyze the correlation be-tween LncRNA NEAT1,peripheral blood LMR.Gensini score and troponin Ⅰ(TnⅠ).ROC curve was used to evaluate the diagnostic efficacy of plasma LncRNA NEAT1 and LMR for ACS.Results There were statistically significant differences in smoking history among the three groups(P＜0.05),and the rate of smoking history in AMI group was higher than that in control group,and the difference was statistically significant(P＜0.017).The levels of creatine kinase isoenzyme MB(CK-MB),TnⅠ and Gensini score in AMI group were higher than those in control group and UA group,and the differences were statistically significant(P＜0.05).The expression level of LncRNA NEAT1 in the AMI group(1.85[1.45,2.31])was higher than that in the control group(0.80[0.35,1.03])and UA group(1.26[0.69,1.38]),and the expression level of LncRNA NEAT1 in the UA group was higher than that in the control group,and the differences were statistically significant(P＜0.05).The level of LMR in AMI group(3.59±1.42)was lower than that in control group(4.97±1.50)and UA group(4.63±1.45),and the differences were statistically significant(P＜0.05).Correlation analysis showed that LncRNA NEAT1 was positively correlated with TnⅠ(r=0.684,P＜0.001),and LMR was negatively correlated with TnⅠ(r=-0.317,P＜0.05).LncRNA NEAT1 was negatively correlated with LMR(r=-0.297,P＜0.05).LncRNA NEAT1 was positively correlated with Gensini score(r=0.654,P＜0.001),and LMR was negatively cor-related with Gensini score(r=-0.393,P＜0.001).ROC analysis showed that the AUC of plasma LncRNA NEAT1 for ACS diagnosis was 0.855(95％CI:0.773-0.936,P＜0.001),and the sensitivity and specificity were 76.4％and 88.0％,respec-tively.The AUC of LMR in the diagnosis of ACS was 0.684(95％CI:0.561-0.807,P＜0.001),and the sensitivity and speci-ficity were 84.7％and 48.0％,respectively.Conclusion Plasma LncRNA NEAT1 expression level and LMR are both asso-ciated with the severity of ACS and coronary artery lesions,which may be used as potential biomarkers to assist the diagnosis of ACS and determine the severity of coronary artery lesions.
Coronary heart diseaseLong non-coding RNANuclear enriched abundant transcript 1Acute coronary syndromeLymphocyte to monocyte ratio
万方数据
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