湖州地区儿童支原体感染横断面调查及耐药突变状况
Epidemiological characteristics of Mycoplasma infection among children in Huzhou area
鲍英 1钱福初 2茅惠莉3
作者信息
- 1. 湖州市中心医院 医院感染管理部,浙江 湖州 313000
- 2. 湖州市中心医院 湖州市感染性疾病精准医学研究与转化重点实验室,浙江 湖州 313000
- 3. 杭州美联医学股份有限公司,浙江 杭州 310014
- 折叠
摘要
目的 调查湖州地区儿童肺炎支原体(Mycoplasma pneumoniae,MP)核酸阳性和耐大环内酯类药物基因突变情况,为临床合理防治MP感染提供依据.方法 回顾性收集湖州地区4家医院的9 631例儿童急性呼吸道感染咽拭子样本,采用real-time PCR技术进行MP核酸及耐药基因(23S rRNA)突变检测,并探讨其流行特征.结果 共检出MP阳性 878 例,阳性率为9.12%.在MP阳性样本中检出耐大环内酯类药物基因23S rRNA突变610 例,耐药突变检出率为 69.48%.男童MP阳性率(8.31%)低于女童(10.05%),差异有统计意义(χ2=8.757,P=0.0301);不同性别儿童耐药突变率差异无统计意义(P>0.05).儿童MP 阳性率随年龄的增长呈上升趋势(χ2趋势=289.46,P<0.001),耐药突变检出率在不同季节差异无统计学意义(χ2=2.662,P>0.05).结论 湖州地区MP感染多发于大年龄段儿童,女童高于男童.大环内酯类耐药突变率较高,需及时监测.
Abstract
Objective To investigate the prevalence of Mycoplasma pneumoniae(MP)nucleic acid positivity and macrolide resistance-associated gene mutations in children with pneumonia in the Huzhou area,in order to provide evidence for the rational prevention and treatment of MP infections in clinical practice.Methods A retrospective collection of 9 631 throat swab samples from children with acute respiratory tract infections in four hospitals in the Huzhou area was conducted.Real-time PCR technology was used to detect MP nucleic acid and macrolide resistance-associated gene mutations(23S rRNA).The epidemiological characteristics of MP were also explored.Results A total of 878 cases(9.12%)were detected with MP positive.Among the MP-positive samples,610 cases were found to have macrolide resistance-associated gene mutations in the 23S rRNA gene,with a detection rate of 69.48%.The positivity rate of MP in boys(8.31%)was lower than that in girls(10.05%),and the difference was statistically significant(χ2=8.757,P=0.0301).However,there was no statistically significant difference in the rate of macrolide resistance-associated gene mutations between different genders of children(P>0.05).The positivity rate of MP in children showed an increasing trend with age(χ2trend=289.46,P<0.001).There was no statistically significant difference in the detection rate of drug-resistant mutations among different seasons(χ2=2.662,P>0.05).Conclusions MP infection is more common in older children in the Huzhou area,with a higher prevalence in girls compared to boys.Moreover,a relatively high rate of macrolide resistance-associated gene mutations was observed.These results highlight the need for timely monitoring of macrolide resistance in MP infections.
关键词
肺炎支原体/耐药/儿童/调查Key words
Mycoplasma pneumoniae/antimicrobial resistance/children/survey引用本文复制引用
基金项目
湖州市公益性应用研究重点项目(2021GZ50)
出版年
2024
国家科技期刊平台
NSTL
万方数据