Objective To investigate the prevalence of Mycoplasma pneumoniae(MP)nucleic acid positivity and macrolide resistance-associated gene mutations in children with pneumonia in the Huzhou area,in order to provide evidence for the rational prevention and treatment of MP infections in clinical practice.Methods A retrospective collection of 9 631 throat swab samples from children with acute respiratory tract infections in four hospitals in the Huzhou area was conducted.Real-time PCR technology was used to detect MP nucleic acid and macrolide resistance-associated gene mutations(23S rRNA).The epidemiological characteristics of MP were also explored.Results A total of 878 cases(9.12%)were detected with MP positive.Among the MP-positive samples,610 cases were found to have macrolide resistance-associated gene mutations in the 23S rRNA gene,with a detection rate of 69.48%.The positivity rate of MP in boys(8.31%)was lower than that in girls(10.05%),and the difference was statistically significant(χ2=8.757,P=0.0301).However,there was no statistically significant difference in the rate of macrolide resistance-associated gene mutations between different genders of children(P>0.05).The positivity rate of MP in children showed an increasing trend with age(χ2trend=289.46,P<0.001).There was no statistically significant difference in the detection rate of drug-resistant mutations among different seasons(χ2=2.662,P>0.05).Conclusions MP infection is more common in older children in the Huzhou area,with a higher prevalence in girls compared to boys.Moreover,a relatively high rate of macrolide resistance-associated gene mutations was observed.These results highlight the need for timely monitoring of macrolide resistance in MP infections.
国家科技期刊平台
NSTL
万方数据
