Objective To investigate the therapeutic mechanism of sulforaphane(SFN)to protect against kidney injury in diabetic mice by regulating AMPK/SIRT1/NF-κB pathway.Methods A total of 30 C57BL/6 mice were randomly divided into a normal control group(10 mice)and an experimental group(20 mice).The latter group was administered a diabetic mouse model via intraperitoneal injection of 50 mg/kg streptozotocin(STZ);the successful model mice were then divided into the model group and the sulforaphane group(each with 10 mice),with the latter receiving daily doses of 0.5 mg/kg sulforaphane.Body weight was measured weekly,and fasting blood glucose was assessed.After 12 weeks of continuous dosing,serum and kidney tissues were collected to test for kidney injury markers,inflammatory factors,and protein levels of the AMPK/SIRT1/NF-κB signaling pathway.Renal pathological damage was observed.High-glucose treated HEK-293T cells were used to replicate the renal cell injury model,and an AMPK inhibitor was added to verify whether SFN exerts anti-inflammatory effects through the activation of the AMPK/SIRT1 pathway.Results In the SFN group,fasting blood glucose levels were reduced to 7.5 mmol/L,insulin levels increased to 0.82 ng/mL,and renal pathological damage was significantly improved.Serum creatinine,blood urea nitrogen,24-hour urinary protein excretion,and pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6),as well as the protein levels of p-p65,were decreased.In contrast,the protein expression levels of p-AMPK and SIRT1 were significantly increased,with all differences being statistically significant compared to the model group(P＜0.05).In vitro experiments indicated that SFN ameliorated high-glucose-induced inflammation in HEK-293T cells by activating the AMPK/SIRT1 signaling pathway and reducing the expression levels of phosphorylated p65 protein.The anti-inflammatory effects of SFN were attenuated by the AMPK inhibitor.Conclusions SFN reduces kidney inflammation by activating AMPK/SIRT1 signaling pathway and inhibiting NF-κB signaling pathway,effectively alleviating kidney injury in diabetic mice.
维普
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