Objective The study aims to analyze the expression of miR-126-3p and miR-210-3p in plasma exosomes of pregnant women with preeclampsia and normal pregnant women in the third trimester,and to investigate their significance in the early prediction and clinical diagnosis of preeclampsia.Methods Medical records of 30 pregnant women with preeclampsia in the late third trimester(observation group)and 30 healthy pregnant women in the late third trimester(control group)were collected.Peripheral venous blood was drawn from the pregnant women,and exosomes and total RNA from plasma exosomes were extracted.The relative expression levels of exosomal miR-210-3p and miR-126-3p were detected by real-time fluorescence quantitative PCR(qRT-PCR).The value of the target miRNA in the clinical diagnosis of preeclampsia was evaluated using the ROC curve analysis.Results The relative expression level of miR-126-3p in the plasma exosomes of pregnant women with preeclampsia(0.74±0.39)was lower than that in the normal pregnancy group(1.05±0.47),and the relative expression level of miR-210-3p(1.72±0.62)was higher than that in the normal pregnancy group(1.35±0.38),with both differences being statistically significant(P＜0.05).The ROC curve analysis showed that the AUC for the diagnosis of preeclampsia using the combined plasma exosomal miR-126-3p and miR-210-3p was 0.775(95％CI:0.629～0.921,P=0.003).Conclusions Compared to healthy pregnant women,the levels of miR-126-3p are decreased and the levels of miR-210-3p are increased in the plasma exosomes of pregnant women with preeclampsia.The combined detection of these two miRNAs shows good diagnostic efficacy for preeclampsia.
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