Objective To analyze the mechanism by which the circRNA001263-miR-214 signaling axis regulates the signaling pathways of nasopharyngeal carcinoma stem cells,providing a reference for exploring new therapeutic targets for nasopharyngeal carcinoma.Methods The expression levels of miR-214 and circRNA001263 mRNA in cancer tissues and adjacent normal tissues from 25 nasopharyngeal carcinoma patients were compared,along with the protein levels of epidermal growth factor(EGF),WNT,Sonic hedgehog(SHH),and bone morphogenetic protein(BMP).An in vitro nasopharyngeal carcinoma stem cell model was established to analyze the effects of circRNA001263 and miR-214 expression on the apoptosis of nasopharyngeal carcinoma stem cells and related proteins.Results The expression levels of miR-214,circRNA001263 mRNA,and EGF,WNT,SHH,BMP proteins in nasopharyngeal carcinoma tissues were all higher than those in adjacent normal tissues,with statistically significant differences(P<0.05).Abnormal expression of circRNA001263 was found in nasopharyngeal carcinoma tissues.In the circRNA001263 mimics group,circRNA001263 NC group,and circRNA001263 inhibitor group,the relative expression levels of circRNA001263 mRNA and miR-214 mRNA,as well as the levels of EGF,WNT,SHH,BMP proteins in nasopharyngeal carcinoma stem cells,showed a decreasing trend,while the apoptosis rate of nasopharyngeal carcinoma stem cells showed an increasing trend;in the miR-214 mimics group,miR-214 NC group,and miR-214 inhibitor group,the relative expression levels of circRNA001263 mRNA and miR-214 mRNA,as well as the levels of EGF,WNT,SHH,BMP proteins in nasopharyngeal carcinoma stem cells,also showed a decreasing trend,with an increasing trend in the apoptosis rate of nasopharyngeal carcinoma stem cells;all differences were statistically significant(P<0.05).Target gene prediction revealed complementary base pairing between circRNA001263 and miR-214.Conclusions Nasopharyngeal carcinoma stem cells regulate EGF,WNT,SHH,and BMP proteins to promote apoptosis through the circRNA001263 and miR-214 signaling pathway.
国家科技期刊平台
NSTL
万方数据
