Objective To observe the effect of electroacupuncture intervention on postoperative cognitive function in aged rats and to explore its potential regulatory mechanisms.Methods A total of 54 18-month-old male SD rats were anesthetized with 2.1%isoflurane.Eighteen rats underwent left paw tibial fracture internal fixation surgery(model group),eighteen rats received electroacupuncture stimulation at the"Baihui""Dazhui"and"Shenting"acupoints after the same surgery(electroacupuncture group),and the remaining eighteen served as the blank group.Water maze experiments were conducted on the 1st day before surgery and the 3rd and 7th days after surgery to observe the changes in spatial cognitive function of rats.After the rats were sacrificed,hippocampal tissue was extracted,and the levels of IL-1β,IL-6,and TNF-α were measured using a colorimetric method.Results On the 7th day after surgery,the escape latency of the model group and electroacupuncture group were(28.8±3.4)s and(22.5±2.2)s,respectively,and the number of times crossing the original platform were(2.9±1.2)and(3.7±0.9),respectively.Both differences were statistically significant(P<0.05).Compared with the model group,the electroacupuncture group showed milder pathological changes in hippocampal neurons,with only a small amount of nuclear pyknosis and dissolution,and the expression levels of IL-6 and TNF-α in the hippocampal tissue were reduced,with statistically significant differences(P<0.05).Conclusions Electroacupuncture can improve the postoperative cognitive function of aged rats and may exert its therapeutic effect by reducing the expression of inflammatory factors IL-6 and TNF-α in the tissue.
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