Curcumin Inhibits Lipogenic Differentiation of 3T3-F442A Preadipocytes by Modulating the p38 MAPK Pathway
Objective:The effects of curcumin on the differentiation and adipogenesis of 3T3-F442A preadipocytes are investigated.Methods:The effects of 0-100 µmol/L curcumin on the proliferation of 3T3-F442A cells at 24,48 and 72 h are detected by MTT assay;3T3-F442A preadipocytes are treated with induced differentiation medium supplemented with 30 μmol/L curcumin,and the effects of curcumin on the adipogenicity of 3T3-F442A preadipocytes are observed by Oil Red O staining method;RT-q PCR,Western blot,and ELISA assay are used to detect the effects of curcumin on the expression of 3T3-F442A preadipocyte differentiation-related transcription factors,adipogenesis-associated vascular endothelial growth factors and receptors,and matrix metalloproteinases expression,phosphorylation levels of p38 MAPK.Results:Curcumin inhibits the proliferation of 3T3-F442A adipocytes,down-regulated the expression levels of adipocyte differentiation-related transcription factors,vascular endothelial growth factors and receptors,and matrix metalloproteinases,and also down-regulates the phosphorylation level of p38 MAPK.Conclusion:Curcumin inhibits 3T3-F442A preadipocyte differentiation and adipogenesis by inhibiting the p38 MAPK signalling pathway and matrix metalloproteinase activity.
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