姜黄素通过调节p38 MAPK通路抑制前脂肪细胞3T3-F442A成脂分化
Curcumin Inhibits Lipogenic Differentiation of 3T3-F442A Preadipocytes by Modulating the p38 MAPK Pathway
程亚青 1刘爱萍 1李佟 1张仁文 1葛雅琨 1张元新1
作者信息
- 1. 吉林化工学院生物与食品工程学院,吉林吉林 132022
- 折叠
摘要
目的:研究姜黄素对前脂肪细胞3T3-F442A分化和脂肪生成的影响.方法:采用MTT法检测0～100 µmol/L的姜黄素在24、48、72 h对3T3-F442A细胞增殖的影响;使用添加30 µmol/L姜黄素的诱导分化培养基处理前脂肪细胞3T3-F442A,通过油红O染色法观察姜黄素对前脂肪细胞3T3-F442A脂肪生成的影响;采用RT-qPCR技术、Western Blot实验、ELISA实验检测姜黄素对前脂肪细胞3T3-F442A分化相关转录因子、脂肪生成相关血管内皮生长因子及受体,以及基质金属蛋白酶表达的影响、p38 MAPK的磷酸化水平的影响.结果:姜黄素能抑制3T3-F442A脂肪细胞增殖,下调脂肪细胞分化相关转录因子、血管内皮生长因子及受体,以及基质金属蛋白酶的表达水平,还下调了 p38 MAPK的磷酸化水平.结论:姜黄素可以通过抑制p38 MAPK信号通路,以及降低基质金属蛋白酶的活性,从而抑制前脂肪细胞3T3-F442A的分化和脂肪生成.
Abstract
Objective:The effects of curcumin on the differentiation and adipogenesis of 3T3-F442A preadipocytes are investigated.Methods:The effects of 0-100 µmol/L curcumin on the proliferation of 3T3-F442A cells at 24,48 and 72 h are detected by MTT assay;3T3-F442A preadipocytes are treated with induced differentiation medium supplemented with 30 μmol/L curcumin,and the effects of curcumin on the adipogenicity of 3T3-F442A preadipocytes are observed by Oil Red O staining method;RT-q PCR,Western blot,and ELISA assay are used to detect the effects of curcumin on the expression of 3T3-F442A preadipocyte differentiation-related transcription factors,adipogenesis-associated vascular endothelial growth factors and receptors,and matrix metalloproteinases expression,phosphorylation levels of p38 MAPK.Results:Curcumin inhibits the proliferation of 3T3-F442A adipocytes,down-regulated the expression levels of adipocyte differentiation-related transcription factors,vascular endothelial growth factors and receptors,and matrix metalloproteinases,and also down-regulates the phosphorylation level of p38 MAPK.Conclusion:Curcumin inhibits 3T3-F442A preadipocyte differentiation and adipogenesis by inhibiting the p38 MAPK signalling pathway and matrix metalloproteinase activity.
关键词
姜黄素/脂肪细胞/细胞分化/脂肪生成/转录因子/基质金属蛋白酶Key words
curcumin/adipocytes/cell differentiation/adipogenesis/transcription factors/matrix metalloproteinase引用本文复制引用
出版年
2024
NETL
NSTL
万方数据