In order to explore the structural characteristics of IGF-1 gene promoter for long character in pigs,the IGF-1 gene 5'UTR was amplified and sequenced from the genomic DNA in four different pig breeds:Tibet Pig,Bama miniature Pig,Northeastern Wild boar and Junmu-1 Pig.With bioinformatics tools,characterization and comparison of these sequences from four varieties of swine were conducted.Results showed that:In IGF-1 gene 5'end,compared with the others,Bama miniature Pig missed a possible CdxA binding site and a possible NKx-2 binding site.Moreover,comparing Junmu-1 Pig with Tibet Pig and Northeastern Wild boar,we found a possible MZF1 binding site in Junmu-1 Pig.The evolutionary tree among four species,constructed by the neighbor-joining method,showed that Tibet Pig had a recently genetic relationship with Bama miniature pig; and a closely genetic relationship with Northeastern Wild boar.And the genetic relationship between Junmu-1 Pig and the others was the farthest.The results show that the structure of IGF-1 gene promoter is different in different body length pigs.
国家科技期刊平台
NSTL
万方数据
维普
