Genetic Expression and Resistance Evaluation of Foreign Genes in Hybrid Lines of Bt Rice
In this study,Bt transgenic rice lines PP-121,PP-123 and PP-136 were crossed with Ji-jing 809,the main rice materials in Jilin province.The hybrid lines of Yj-276,Yj-231 and Yj-288 were obtained and the breeding value was evaluated through genetic expression and resistance evalu-ation of foreign genes.The results of genetic analysis showed that foreign genes of cry1C and bar were linked and co-segregated in the hybrid progenies.Enzyme Linked Immunosorbent Assay(ELISA)showed that cry1C protein was expressed normally in hybrid lines,up to 1.78 μg/g.Insect and herbicide resistance evaluation showed that three hybrid lines were highly resistant to striped stem borers and Basta 1.0 × 10-3 mol/L compared to female parents.Agronomic character analysis showed that some hybrid progenies had better performance than that of Jijing809.This study pro-vides new germplasm resources and a theoretical basis for insect-resistant rice breeding.
万方数据
维普
