吉林农业大学学报2024,Vol.46Issue(1) :34-39.DOI:10.13327/j.jjlau.2020.5574

玉米茎腐病病原菌Fusarium graminearum拮抗真菌的筛选与鉴定

Screening and Identification of Antagonistic Fungi against Fusarium graminearum,a Pathogen of Corn Stalk Rot

赵子健 王瑶 张逸凡 邓颖 张丹 王晓梅
吉林农业大学学报2024,Vol.46Issue(1) :34-39.DOI:10.13327/j.jjlau.2020.5574

玉米茎腐病病原菌Fusarium graminearum拮抗真菌的筛选与鉴定

Screening and Identification of Antagonistic Fungi against Fusarium graminearum,a Pathogen of Corn Stalk Rot

赵子健 1王瑶 1张逸凡 1邓颖 1张丹 1王晓梅1
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作者信息

  • 1. 吉林农业大学植物保护学院,长春 130118
  • 折叠

摘要

针对吉林省玉米茎腐病的主要优势致病菌禾谷镰孢菌进行拮抗菌株的筛选和鉴定,为玉米茎腐病生防菌剂的研制奠定基础.从300份土壤中分离出685个菌株,选取生长势较好的212个菌株,分别与禾谷镰孢菌进行平板对峙试验,筛选出拮抗效果较好的拮抗菌株5株.将这5株菌进行抑菌试验,其中M-43号菌株抑菌率最高,为75.71%,选取M-43号真菌菌株进行后续研究.基于形态学显微观察和ITS序列分析,鉴定M-43号菌株为Talaromyces pinophilus,该种菌为吉林省新纪录种.

Abstract

Screening and identification of antagonistic strains against Fusarium graminearum,the main dominant pathogen of corn stalk rot in Jilin province,lays the foundation for the development of biocontrol agents for corn stalk rot.The experimental results are as follows:685 strains were isolated from 300 parts of soil,and 212 strains with good growth potential were selected to conduct a plate-to-plate test with Fusarium graminearum,and 5 antagonistic strains with better antagonistic effect were selected.Five strains were tested for bacteriostasis.Among them,the bacteriostasis rate of M-43 strain was 75.71%,and the bacteriostasis rate was the highest.M-43 fungus strain was selected for subsequent experimental research.Based on morphological microscopy and ITS sequence analysis,strain M-43 was identified as Talaromyces pinophilus,and this species was a new record species in Jilin province.

关键词

玉米茎腐病/拮抗菌株/Fusarium/graminearum/Talaromyces/pinophilus

Key words

corn stalk rot/antagonistic bacteria/Fusarium graminearum/Talaromyces pinophilus

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基金项目

国家重点研发计划项目(2016YFD0201004)

国家重点研发计划项目(2016YFD0200708)

国家重点研发计划项目(2018YFD0201200)

吉林省科技发展计划项目(20200404015YY)

出版年

2024
吉林农业大学学报
吉林农业大学

吉林农业大学学报

CSTPCDCSCD北大核心
影响因子:1.014
ISSN:1000-5684
参考文献量28
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