Disease Resistance Mechanism of Pinus koraiensis Induced by Bacil-lus amyloliquefaciens AW3
The aim of the present study was to investigate the effect of plant growth-promoting rhizo-bacteria(PGPR)Bacillus amyloliquefacien AW3 strain on disease resistance in Pinus koraiensis in-fected with Fusarium oxysporum.P.koraiensis saplings were inoculated with AW3 using fermenta-tion liquor under greenhouse conditions.Relative control factors of root rot such as soluble protein,soluble sugar,phenylalanine ammonia lyase(PAL),catalase(CAT),superoxide dismutase(SOD)and disease resistance related genes were determined.The results indicated that the inhibitory effect of AW3 on root rot in P.koraiensis was remarkably high(79.3％).AW3 induced a rapid generation of cell defense in P.koraiensis after inoculation of AW3 in P.koraiensis.The relative staining intensity of reactive oxygen species(ROS)was 2.57 and cell membrane permeability was 2.98 times that of the control group,and the difference was significant(P＜0.05).The content of soluble sugar and suluble protein of P.koraiensis saplings increased significantly(P＜0.05).AW3 significantly increased the ac-tivity of SOD,POD and reduced CAT.AW3 also induced transcription of jasmonic acid(JA),sali-cylic acid(SA),and genes involved in auxin signaling.In summary,AW3 induced disease resistance against F.oxysporum infection in P.koraiensis and reduced occurrence of the disease through a com-bined action of defense-related enzymes and resistance genes.AW3 is a very effective bacterial strain that can be used to control root rot in P.koraiensis,however further studies on the development of biologics using AW3 and its field applications are advocated.
biocontrolbacillusinduction of disease resistancePinus koraiensisroot rot disease
万方数据
维普
