Rapid Propagation Technology of Seedlings from Protocorm of Oreor-chis patens(Lindl.)Lindl.
Using protocorm produced by one-year-old corm as explant and MS as basic culture me-dium,the effects of four auxins and mitogens on the proliferation and differentiation of protocorm were studied,and together studied were the effects of hormone combinations on the branching elonga-tion,proliferation and differentiation of protocorm,as well as the production of corm and rooting of corm during protocorm differentiation.The results showed that NAA and ZT were beneficial to the pro-liferation of protocorm by branching elongation,and the proliferation coefficient reached 8.67 at NAA 1.0 mg/L+ZT 1.0 mg/L.IAA and 6-B A were beneficial to the differentiation of protocorm into corm,and the differentiation coefficient reached 5.14 at IAA 2.5 mg/L+6-BA 2.5 mg/L.IAA and 6-BA were ben-eficial to the rooting of corm formation from protocorm,and the number of roots reached 6.26 per corm and the weight reached 9.90 mg per root at IAA 2.5 mg/L+6-BA 0.1 mg/L.
万方数据
维普
