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罗汉果根的生物活性及化学成分

Chemical Constituents from the Root of Siraitia grosvenorii and Their Biological Activities

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以受根结线虫危害的罗汉果根为研究对象,研究不同极性部位的生物活性及化学成分.罗汉果根粗提物过HPD-100大孔树脂层析柱,依次用不同浓度甲醇梯度洗脱,收集洗脱液,得到Fr1,Fr2,Fr3,Fr4部位,利用传统分离方法和反相制备色谱技术进行分离纯化,采用ESI-MS,NMR对化合物进行结构鉴定;以抑制α-葡萄糖苷酶和HepG2肿瘤细胞能力为指标,研究各极性部位和部分化合物的生物活性.结果表明:Fr4部位具有最强的抑制α-葡萄糖苷酶和HepG2肿瘤细胞增殖活性.从Fr4中分离得到4种葫芦素,分别为双氢葫芦素E(1),葫芦素E(2),二氢异葫芦素B-25-乙酸酯(3),葫芦素B(4),化合物均为首次从罗汉果属中分离得到,且化合物4有较好的抗肿瘤活性.
To study the biological activities and chemical constituents of Siraitia grosvenorii roots,the crude extracts of S.grosvenorii roots were loaded to HPD-100 column and eluted by gradient methanol-water solutions to obtain Fr1,Fr2,Fr3 and Fr4 fractions.Traditional separation methods and reversed-phase preparative chromatography techniques were used for separation and purifica-tion,and ESI-MS and NMR were used for identifying their structure.α-glycosidase and HepG2 tu-mor cell inhibition activity of different polar parts and partical compounds were determined.The re-sults indicate that Fr4 has the highest α-glycosidase and HepG2 tumor cell inhibition activity.Four compounds were isolated from Fr4,and their structures were identified as dihydrocucurbitacin E,cu-curbitacin E,dihydroisocucurbitacin B-25-acetate and cucurbitacin B,and they are isolated from S.grosvenorii for the first time.

Siraitiae grosvenorii rootbiological activitycucurbitacinα-glycosidaseHepG2 tu-mor cell

扈芷怡、卢凤来、赵立春、颜小婕、徐祥林、李典鹏

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广西中医药大学药学院,南宁 530011

广西壮族自治区中国科学院广西植物研究所,桂林 541006

广西中医药大学第一附属医院,南宁 530023

罗汉果根 生物活性 葫芦素 α-葡萄糖苷酶 HepG2肿瘤细胞

广西自然科学基金项目广西中医药大学"岐黄工程"高层次人才团队培育项目桂林市重大专项广西重点研发计划项目国家自然科学基金项目广西植物研究所基本业务费

2017GXNSFAA198098201800220170303桂科AB1638010821562009桂植业18003

2024

10.13327/j.jjlau.2020.5596

吉林农业大学学报
吉林农业大学

吉林农业大学学报

CSTPCD北大核心
影响因子：1.014
ISSN：1000-5684
年,卷(期)：2024.46(1)
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