Changes of Phenolic Acid Content and Enzyme Activity in Different Potted Ginseng Soils
Ginseng continuous cropping obstacle is a major industrial problem which restricts the sustainable development of ginseng industry and has not been effectively solved so far.Phenolic acid compound is one of the main allelochemicals of ginseng continuous cropping obstacles.Using new forest soil without ginseng cultivation as control(ck),this study investigated the dynamic changes in phenolic acid content and enzyme activity of different potted ginseng soils at different growth stages under five treatments:old ginseng soil(L1),new forest land(X1),rhizosphere soil(G1),new forest soil mixed with rotten stems and leaves(X2)and rhizosphere soil mixed with rotten stems and leaves(G2)five phenolic acids content of soil in different growth period and the enzyme activity of dynamic change,so as to provide theoretical basis for analyzing the causes of ginseng continuous cropping ob-stacles.HPLC and enzyme activity assay were used to analyze changes in phenolic acid content and soil enzyme activity in different potted ginseng soils at different growth stages and to monitor the aboveground and underground growth of different potted ginseng.The results showed that:four phe-nolic acids were detected in different potted ginseng soils.The contents ranged from high to low,in-cluding benzoic acid,salicylic acid,cinnamic acid and gallic acid.With the extension of cultivation time,acid content in soil showed an increasing trend.There were significant differences in soil en-zyme activities of different potted ginseng plants,with the activity of sucrase increasing first and then decreasing in each group.The lacease activities of L,and X2 groups showed an upward trend,X1 and G1 groups showed a downward trend,and G2 group showed a downward trend first and then an up-ward trend.Catalase activity in groups L1,X2 and X,decreased first and then increased,while that in groups G1and G2 increased.Acid phosphatase activities in L1,X,and G1 groups showed an upward trend,while those in X2 and G2 groups showed an upward trend and then a downward trend.The ure-ase activity of G(group showed a decreasing trend,while that of L1,X1,G1 and X2 groups showed a decreasing trend and then an increasing trend.The aboveground and underground roots of group X1 had the best growth without disease spots,while the aboveground and underground growth of group G2 was inhibited,and the growth rate of X,root weight was significantly higher than that of L1,X2,G,and G2.We speculated that soil enzyme activities are closely related to root secretion,plant residue shedding and metabolites of pathogenic microorganisms,which may affect soil enzyme activity by af-fecting the accumulation of phenolic acids in soil,thereby affecting the absorption and utilization of nutrients in ginseng,and ultimately leading to continuous cropping obstacles in ginseng.
万方数据
维普
