After primary isolation and identification of antler mesenchymal stem cells(AMSCs),exo-somes were isolated from AMSCs culture medium by UC(Ultracentrifugation)and exoEasy methods.The effects of AMSCs-Exo extracted with two methods were compared,and the functions of AMSCs-Exo were verified.The results showed that AMSCs-Exo could be isolated by both UC method and exoEasy method.The isolated AMSCs-Exo by UC method had more complete structure,fewer impuri-ties,and more representative particle size distribution,compared with AMSCs-Exo isolated by exoEasy method.It had a single main peak and AMSCs-Exo protein marker could be detected by WB(Western blot).The isolated AMSCs-Exo by exoEasy method had lower concentration,more incom-plete structure,more impurities,more dispersed particles,and more unrepresentative distribution,compared with AMSCs-Exo isolated by UC method.It had no single main peak and no detectable pro-tein marker.EdU results showed that AMSCs-Exo could promote the proliferation of haCat cells sig-nificantly.By comparing the two methods,UC method is more suitable for relevant functional verifi-cation studies.
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