Inhibitory Effects of Desmodium Caudatum Extracts on H1N1 Influ-enza A Virus Infection in Vitro
The maximum safe concentration(MNTC)of Desmodium caudatum extracts(DCE)on MDCK cells was determined by CCK8 method.The model of MDCK cells infected with influenza vi-rus A/PR8/34(H1N1,PR8)was established,and its inhibitory effect on cytopathic changes caused by viral infection was detected using CCK8 method.Using fluorescence quantitative PCR method,the ef-fects of DCE on influenza virus genes,proteins and expression of host inflammatory transcription fac-tors and inflammatory cytokines were detected.The cytotoxicity test results showed that the MNTC of DCE on MDCK cells was 100 mg/L.The qRT-PCR results showed that DCE could significantly re-duce mRNA expression of M and NP genes of PR8 influenza virus(P＜0.01),and also significantly downregulated the expression levels of inflammatory transcription factors NF-κB p65 and STAT3,and inflammatory cytokines IL-1β and TNF-α,whose inhibitory effect was positively correlated with drug concentration.Immunoblotting assay showed that DCE of high concentration(100 mg/L)sig-nificantly inhibited the expression of influenza virus M1 protein(P＜0.01).These results indicate that Desmodium caudatum extracts have significant in vitro anti-influenza virus effects,and lays foundation for identifying the exact pharmacological components with anti influenza virus effects from Desmodium caudatum.
influenza A virusDesmodium caudatum extractCCK8antiviral effectinflamma-tory cytokine
万方数据
维普
