Prokaryotic Expression and Polyclonal Antisera Preparation of Vacu-olar Invertase in Potato
Potato vacuolar invertase plays a key role in potato cold-induced sweetening.In this study,N-terminal fragment of potato vacuolar invertase StVIN1 gene was inserted into the prokaryotic expression vector pET-28a,and the recombinant plasmid pET-28a-StVIN1-N was transformed into the host E.coli BL21(DE3)by heat shock.The fusion protein with 6 × His tag was induced by 0.84 mmol/L IPTG.Rabbits were immunized with the purified protein as an antigen to obtain poly-clonal antisera.The obtained polyclonal antisera was used to identify the purified protein by Western blot.The results showed that the prepared polyclonal antisera has good specificity.
万方数据
维普
