Transcriptome Analysis of Two Resistant and Susceptible Tobacco Varieties in Response to Early Potato Y Virus Stress
In order to explore the response differences of different tobacco resistant varieties NC95 and SY04-3 to potato virus Y stress,tobacco seedlings were inoculated with PVY,and seedling samples were collected at 12 h.Transcriptome sequencing and bioinformatics analysis were per-formed.The analysis results of PVY inoculation and control showed that NC95 up-regulated 206 dif-ferentially expressed genes and down-regulated 111 genes.SY04-3 up-regulated 1 001 differentially expressed genes,down-regulated 423.The functions of these differentially expressed genes are classi-fied into 54 GO entries in three categories:biological processes,cell components and molecular func-tions.NC95 and SY04-3 up-regulated differentially expressed genes were significantly enriched in 7 KEGG metabolic pathways,while NC95 and SY04-3 down-regulated differentially expressed genes were significantly enriched in 10 and 14 KEGG metabolic pathways,respectively;Transcription fac-tors predicted that 20 transcription factors in 13 families of NC95 changed significantly,and 38 tran-scription factors in 20 families of SY04-3 changed significantly.This study provides reference for the study on the early response of tobacco to potato Y virus infection mechanism.