Transcriptional Expression Analysis of GMC Gene Family from Lenz-ites gibbosa under Woody Condition
In order to reveal the function of glucose-methanol-choline oxidoreductases(GMCs)genes of Lenzites gibbosa(Trametes gibbosa)during wood decomposition,transcriptome sequencing was per-formed on L.gibbosa mycelia at 0,3,5,7 and 11 days during the decomposition of wood chips by high-throughput sequencing technology.Count value and corrected FPKM value of each gene were used as expression levels to screen genes and differentially expressed genes(DEGs)between the samples of non-woody control group and the wood-treated samples at 3,5,7 and 11d.The changes of gene expression levels and the annotated results in function of genes in eggNOG,COG,GO,KEGG,NR,SwissProt and Pfam databases were analyzed.The expression of five important DEGs was veri-fied by fluorescence quantitative PCR(qPCR).A total of 23 GMC genes were screened based on the gene annotation results,and the change of expression showed that 9 of them were DEGs.Functional annotation and enrichment results showed that at least 6 kinds of GMCs in L.gibbosa,namely cellobi-ose dehydrogenase,glucose oxidase,pyranose dehydrogenase,L-sorbose 1-dehydrogenase,alcohol oxidase and pyranose oxidase,belonged to four subfamilies of CAZy-AA3 family.In the process of wood lignin and holocellulose degradation,they were mainly involved in carbon metabolism,methane metabolism,amino acid metabolism and lipid metabolism related to energy transport and metabo-lism.Various GMCs had a specific relationship with the formation of H2O2 during lignin and cellu-lose degradation.They could bind with flavin adenosine dinucleotide(FAD)to oxidize the primary al-cohol group to form aldehyde and release H2O2,providing a constant reaction substrate for peroxi-dases.Due to their important auxiliary role in the degradation of lignin and cellulose,GMCs are im-portant auxiliary enzymes related to wood degradation.This study provides a basis for the functional study of GMC family in wood degradation of white rot fungi.
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