Diversity of Rhizosphere Microbial Community of Different Age Panax ginseng in Natural Broad-leaved Secondary Forests
To explore the community composition and distribution patterns of bacteria and fungi in the rhizosphere of Panax ginseng under natural broad-leaved secondary forests,taking ginseng rhizo-sphere soil with different growth years as the research object,Illumina HiSeq high-throughput se-quencing technology was used to analyze the microbial community diversity of ginseng rhizosphere soil at different growth years in natural broad-leaved secondary forest.The results showed that the number of OTUs of bacteria and fungi generally increased with the ginseng planting years.Among them,the number of OTUs of 11-year-old ginseng rhizosphere microorganisms was the largest.The α diversity analysis showed that compared with the soil without planting ginseng,the richness and di-versity of soil bacterial communities with growth years of 7 years(K7)and 11 years(K1 1)were sig-nificantly increased(P<0.05),and there was no significant difference in α diversity index of bacterial communities with 15 years,while fungi richness and diversity indexes of the community did not change signifcantly with the growth years of ginseng.β diversity analysis showed that the rhizosphere bacterial and fungal communities of the same year-old ginseng were relatively close.Among them,the 15-year-old ginseng rhizosphere bacterial and fungal communities were completely separated from the control samples,indicating that the community differences were significant.The abundance of dominant bacteria phyla changed with the increase of ginseng age.The number of Actinomycetes changed significantly with the planting years of ginseng,showing a first increasing and then decreas-ing trend.The number of Actinomycetes in the 4-year-old ginseng reached the maximum value.The abundance of dominant fungi phyla changed with the increase of ginseng age.Under the natural broad-leaved secondary forest,the microbial community of ginseng rhizosphere under the broad-leaved forest changed significantly with the growth of ginseng,which can provide a theoretical basis for the scientific planting of ginseng under the forest.