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天然阔叶次生林下不同年生人参根际微生物群落多样性

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为探讨天然阔叶次生林下人参根际细菌和真菌的群落组成和分布规律,以不同生长年限人参根际土壤为研究对象,采用Illumina HiSeq高通量测序技术分析天然阔叶次生林下不同生长年限人参根际微生物菌群落多样性。结果表明:人参根际细菌和真菌的OTU数目,随着人参生长年限的增长普遍增加,其中,11年生(K11)人参的根际微生物OTU数目最多。α多样性分析结果表明,相对于未种植人参土壤(Kck),生长年限分别为7年(K7)和11年(K11)的土壤细菌群落的丰富度和多样性明显增加(P<0。05),15年(K15)的细菌群落α多样性指数没有明显差异,而真菌群落的丰富度和多样性指数,随着人参的生长年限变化不大。β多样性分析表明,相同年生人参根际细菌和真菌群落距离较近,其中,15年生(K15)人参根际细菌和真菌群落与对照(Kck)样本完全分开,群落差异明显。优势细菌门的相对丰度随着人参生长年限的增长发生改变。放线菌门数量随着种植年限有大幅变化,呈现先升高后降低的趋势,4年生(K4)数量达到最大值。优势真菌门的相对丰度随着人参生长年限的增长发生改变。天然阔叶次生林下,生长年限对人参根际土壤微生物有较大影响,可为林下人参科学种植提供理论依据。
Diversity of Rhizosphere Microbial Community of Different Age Panax ginseng in Natural Broad-leaved Secondary Forests
To explore the community composition and distribution patterns of bacteria and fungi in the rhizosphere of Panax ginseng under natural broad-leaved secondary forests,taking ginseng rhizo-sphere soil with different growth years as the research object,Illumina HiSeq high-throughput se-quencing technology was used to analyze the microbial community diversity of ginseng rhizosphere soil at different growth years in natural broad-leaved secondary forest.The results showed that the number of OTUs of bacteria and fungi generally increased with the ginseng planting years.Among them,the number of OTUs of 11-year-old ginseng rhizosphere microorganisms was the largest.The α diversity analysis showed that compared with the soil without planting ginseng,the richness and di-versity of soil bacterial communities with growth years of 7 years(K7)and 11 years(K1 1)were sig-nificantly increased(P<0.05),and there was no significant difference in α diversity index of bacterial communities with 15 years,while fungi richness and diversity indexes of the community did not change signifcantly with the growth years of ginseng.β diversity analysis showed that the rhizosphere bacterial and fungal communities of the same year-old ginseng were relatively close.Among them,the 15-year-old ginseng rhizosphere bacterial and fungal communities were completely separated from the control samples,indicating that the community differences were significant.The abundance of dominant bacteria phyla changed with the increase of ginseng age.The number of Actinomycetes changed significantly with the planting years of ginseng,showing a first increasing and then decreas-ing trend.The number of Actinomycetes in the 4-year-old ginseng reached the maximum value.The abundance of dominant fungi phyla changed with the increase of ginseng age.Under the natural broad-leaved secondary forest,the microbial community of ginseng rhizosphere under the broad-leaved forest changed significantly with the growth of ginseng,which can provide a theoretical basis for the scientific planting of ginseng under the forest.

broad-leaved forestPanax ginsengrhizosphere microorganismhigh-throughput se-quencing technology

刘丽娟、佟爱仔、秦佳梅

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通化师范学院生命科学学院,通化 134001

吉林省长白山生物种质资源评价与应用重点实验室,通化 134001

阔叶林 人参 根际微生物 高通量测序技术

吉林省科技发展计划

20190304012YY

2024

吉林农业大学学报
吉林农业大学

吉林农业大学学报

CSTPCD北大核心
影响因子:1.014
ISSN:1000-5684
年,卷(期):2024.46(2)
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