Effect of Arginyl-Fructosyl-Glucose on Melanoma B16 Cells and Its Mechanism
To observe the inhibition of Arginyl-Fructosyl-Glucose(AFG)on the proliferation and apoptosis of melanoma B16 cells,and discuss the mechanism of its Bcl-2,Caspase-3 and Bax signal-ing pathways preliminarily,melanoma B16 cells were cultured.1,2.5,5,10,25,50,75 and 100 mol/L of Arginyl-Fructosyl-Glucose were prepared to act on melanoma B16 cells.CKK-8 method was used to detect the effect of Arginyl-Fructosyl-Glucose on the proliferation of B16 cells after 48h;The expression level of LDH was detected by LDH after treatment;The effect of Arginyl-Fructosyl-Glucose on apoptosis of B16 cells was observed by Hoechst 33258 fluorescence staining;RT-PCR and Western Blot were used to analyze the effect of Arginyl-Fructosyl-Glucose on the expression of Bcl-2,Caspase-3,Bax mRNA and protein in B16 cells.With the increase of drug concentration and culture time,the inhibition rate of Arginyl-Fructosyl-Glucose on B16 cells increased,and the inhibi-tion rate reached a plateau after 48 h.The inhibitory effect of Arginyl-Fructosyl-Glucose at 50,75 and 100 mol/L concentrations was the best,and the survival rate of B16 cells was significantly re-duced(P＜0.05,P＜0.01).RT-PCR and Western Blot assay showed that protein levels and Bcl-2 mRNA in B16 cells in the 75 and 100 mol/L groups significantly decreased(P＜0.01),and Caspase-3 mRNA and protein levels significantly increased(P＜0.01).Arginyl-Fructosyl-Glucose can in-hibit the proliferation of melanoma B16 cells and induce their apoptosis.The mechanism may be re-lated to the upregulation of Bcl-2,Caspase-3 and the downregulation of Bax.
Arginyl-Fructosyl-Glucosemelanoma B16 cellproliferation and apoptosismalig-nant tumorginseng
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