LEC-5在秀丽线虫中的表达及亚细胞定位
Expression and Subcellular Localization of LEC-5 in Caenorhabditis elegans
张楠 1程淑琴 1宫鹏涛 1李建华 1王晓岑 1李新 1张西臣1
作者信息
- 1. 吉林大学动物医学学院人兽共患病研究所,人兽共患病研究教育部重点实验室,长春 130062
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摘要
采用gibson组装法构建带有lec-5启动子的lec-5p::gfp::lec-5::lec-5 3'UTR目的质粒和带有肠道特异性vha-6启动子的vha-6p:-gfp::lec-5::lec-5 3'UTR目的质粒,并通过显微注射方法获得游离型的LEC-5P::GFP::LEC-5荧光蛋白标记虫株和整合型的VHA-6P::GFP::LEC-5荧光蛋白标记虫株.利用激光共聚焦显微镜观察LEC-5在秀丽线虫中的表达和亚细胞定位.结果表明:LEC-5在秀丽线虫的肌肉组织和表皮组织中表达,并位于肠上皮细胞的顶端膜和胞质中的囊泡上.研究结果为进一步深入研究LEC-5在RAB-11介导的上皮细胞极性调控中的作用提供试验依据.
Abstract
The lec-5p::gfp::lec-5::lec-5 3'UTR target plasmid with lec-5 promoter and the vha-6p::gfp::lec-5::lec-5 3'UTR target plasmid with intestinal specific vha-6 promoter were constructed by gibson assembly method.Then,the extrachromosomal strain LEC-5P::GFP:LEC-5 and the inte-grated strain VHA-6P::GFP::LEC-5 were obtained by microinjection.The expression and subcellu-lar localization of LEC-5 in Caenorhabditis elegans were observed by confocal microscopy.The re-sults showed that LEC-5 was expressed in muscle and epidermal cells of Caenorhabditis elegans and was located at the apical membrane and cytoplasmic vesicles in the intestinal epithelial cells.These results will lay foundation for further study of the role of LEC-5 in RAB-11-mediated epithelial membrane polarity regulation.
关键词
秀丽线虫/LEC-5蛋白/GFP标记虫株构建/表达/亚细胞定位Key words
Caenorhabditis elegans/LEC-5 protein/GFP-labeled strain construction/expression/subcellular localization引用本文复制引用
基金项目
吉林省科技发展计划(20190103075JH)
出版年
2024
国家科技期刊平台
NSTL
万方数据