Molecular Cloning,Transcriptional Activation Activity and Subcellu-lar Localization of GmTGA15 Gene from Glycine max
GmTGA15 gene was cloned by reverse transcription PCR(RT-PCR)from soybean"Wil-liams 82".Bioinformatics analysis shows that the genome sequence of GmTGA15 includes 8 exons and 7 introns and GmTGA15 contains a complete open reading frame(ORF)of 1 089 bp encoding of 362 amino acid residues.Analysis of the physicochemical properties of the GmTGA15 protein shows that the molecular mass was 41.01 kDa,and the theoretical isoelectric point is 6.28,which is a hydro-philic protein;The primary structure of the GmTGA 15 protein has a typical bZIP conserved domain,belonging to the family of bZIP transcription factors.Homologous alignments and phylogenetic analy-sis of amino acid sequence show that GmTGA15 protein and TGA proteins of 15 other plants all have the same bZIP conserved domain.GmTGA15 protein is most closely related to wild soybean GsTGA,and the consistency of sequence alignment is 97.25％.Secondary structure prediction of GmTGA15 shows that α-helices are dominated,accounting for 63.81％.GmTGA15 possessed transcriptional ac-tivation activity by the method of a yeast one-hybrid assay.The fusion expression vector was intro-duced into Arabidopsis protoplasts by a PEG4000-mediated method and the results showed that Gm-TGA15 was localized in the nucleus.TGA transcription factors play an important role in plants'resis-tance to stress and growth.The results of this study can provide basis for further exploration of the function of GmTGA15 gene.Meanwhile,it can provide theoretical reference for study on plant stress-resistant regulation mechanism in the future.
万方数据
维普
