Effects of EMS Mutation on Flowering Period of Maize and Its Identi-fication Analysis
In order to explore the impact of changes in maize flowering period on growth conditions in different developmental stages,we used methyl sulfonyl ethyl(EMS)mutagenesis technology to process the seeds of maize superior inbred line D12164,and screened the early maize flowering mu-tant M12164.The determination included the expression levels of genes related to development at jointing stage,the photosynthetic rate at different growth stages,the rate of dry matter accumulation,the nutrient content of corn kernels,drought resistance at the seedling stage,glufosinate resistance and Mendelian genetic analysis.The results showed that,compared with the control D12164,the mu-tant M12164 had a high expression of genes related to photosynthesis and cell breakdown during the jointing stage.The photosynthetic rate increased in different periods,and the dry matter accumula-tion was faster;The content of grain protein,starch and fat was increased;Drought resistance de-creased,and glufosinate tolerance increased;The Mendelian genetic segregation ratio was 3∶1,indi-cating that the early flowering phenotype is inherited in a recessive way.Therefore,the results of this study provide technical support for the cloning of genes controlling the early flowering of maize,the molecular design breeding and improvement of flowering period.
万方数据
维普
