The Pleurotus ostreatus versatile peroxidase gene was transferred into Pichia pastoris strain X33 and expression was achieved.The enzymatic properties of recombinant yeast were deter-mined and its ability to degrade corn stover lignin was determined.The results showed that the opti-mal pH values for the decomposition of 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid,2,6-dimethoxyphenol,2-methoxyphenol and veratryl alcohol by recombinant versatile peroxidase were 2.5,3,3.5 and 4.5,respectively.Except for the optimum reaction temperature for decomposition vera-tryl alcohol,which was 40 ℃,the optimum temperature of other substrates was 50 ℃,and it had good thermal stability and acid and alkali resistance.The degradation rates of lignin in corn stover by re-combinant multifunctional peroxidase and by native Pleurotus ostreatus were 23.59％and 10.93％,re-spectively.The results provide a theoretical basis for studying the mechanism of lignin degradation by versatile peroxidase.
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