Effects of Grape Pruning Addition on Soil Fungal Community Struc-ture and Grape Tree Growth
Pot experiment was conducted to explore the effects of grape pruning addition on the growth of grape tree and fungal structure,and to evaluate the feasibility of recycling grape prunings.Grape prunings were added as growing media in different proportions to one-year-old"Shine Mus-cat"grapes to determine the changes of plant shoot length,shoot diameter,leaf area,and total root volume.At the same time,high-throughput sequencing technology was used to study the changes of soil fungal community structure.At the phylum level,Basidiomycota and Ascomycota belonged to the dominant group.The treatments with different proportions of grape prunings reduced the relative abundances of Alternaria,Preussia,Acremonium,Papulaspora,Neocosmospora,Bipolaris,Chaeto-mium,Acaulium,Cladosporium,and Diaporth after 60,120 and 150 days.Among them,T2 treatment also reduced the relative abundance of Stachybotrys,Acrocalymma,Albifimbria,Gibellulopsis,and As-pergillus throughout the experimental period,and increased the relative abundance of Psathyrella at 60,90 and 150 days.It was found that proper proportions of grape prunings could increase shoot length and total root volume of grape plants.Among them,shoot length in T2 treatment was 1.2 times that of the control group after 150 days.In a word,appropriate addition of grape prunings optimized the soil fungal community structure,promoted the degradation of lignin,and formed a soil that inhib-ited pathogenic fungi,which would reduce the risk of grape infection.Among them,T2 treatment had the best comprehensive performance in improving soil fungal community structure and promoting tree growth.
grape pruningbranch returning to the fieldsoil microorganismhigh-throughput se-quencing
万方数据
维普
