Proteomics Analysis of MRb1 on Skeletal Muscle in Type 2 Diabetic Mice Induced by High-fat Diet Combined with Streptozotocin
The purpose of this study was to explore the therapeutic effect of malonyl ginsenoside MRb1 on type 2 diabetic mice,and to further study its action mechanism through the analysis of skel-etal muscle proteomics.In the experiment,C57BL/6J mice were selected as the research object,and type 2 diabetes mouse model was established by high-fat diet combined with streptozotocin.Normal group(NC),model group(DC),MRb1 high dose group(MRb1-40)and MRb1 low dose group(MRb1-20)were set up,with 10 rats in each group.During the experiment,mice in the high-dose and low-dose MRb1 groups were given a 40 mg/(kg·d)and 20 mg/(kg·d)MRb1 for 5 weeks.The results showed that MRb1 could significantly reduce fasting blood glucose,blood lipid levels and homeostatic model assessment-insulin resistance indexes,increased serum insulin levels and alleviate insulin resistance and hyperlipemia in type 2 diabetic mice.Meanwhile,the results of skeletal muscle proteomics showed that 108 differentially expressed proteins were screened between NC group and DC group,in-cluding 62 up-regulated proteins and 46 down-regulated proteins.The KEGG signaling pathways en-riched by differentially expressed proteins mainly include PPAR signaling pathway,fatty acid degra-dation,fatty acid metabolism,insulin signaling pathway,AMPK signaling pathway,and so on.In ad-dition,a total of 48 differentially expressed proteins were screened between DC group and MRb1 group,of which 39 were up-regulated and 9 were down-regulated.The KEGG signaling pathways en-riched by differentially expressed proteins mainly include glycolysis,gluconeogenesis,glucagon sig-naling pathway,calcium signaling pathway,and so on.These results showed that the administration of MRb1 significantly alters protein expression profile in the skeletal muscle of diabetic mice and af-fects multiple signaling pathways closely related to diabetes and skeletal muscle.This study provides a reference for the screening of diabetic skeletal muscle-related pathogenesis and therapeutic targets.
万方数据
维普
