Amelioration of Antioxidative Fraction from Buckwheat Hulls against Oxidative Stress in HepG2 Cells
Buckwheat hulls are rich in flavonoids with multiple functional activities.Seven fractions were obtained by isolation and purification.Five antioxidative fractions were screened by scavenging activities for hydroxyl(·OH)radicals,superoxide anion(O2·-)radicals and 1,1-diphenyl-2-trinitro-phenylhydrazine(DPPH·)radicals,and total antioxidant capacity(T-AOC).The amelioration of these five fractions on the oxidative damage of HepG2 cells induced by H2O2 and high glucose was carried out.The results showed that in the H2O2-induced oxidative damage model of HepG2 cells,PBHE-M4 significantly increased cell viability,which was(8.69±4.04)％and(22.66±7.61)％higher than those of rutin and ascorbic acid,respectively.In the high glucose-induced oxidative damage model of HepG2 cells,all five fractions significantly improved cell viability compared with rutin and ascorbic acid,and PBHE had the best effect in improving cell viability,which was significantly increased(30.14±2.83)％and(36.65±4.12)％over rutin and ascorbic acid,respectively.The changes of the oxi-dative stress factor indicated that the amelioration of five fractions on cell activity was related to intra-cellular antioxidant effects,including increasing the activities of peroxidase(POD),catalase(CAT)and total antioxidant capacity(T-AOC)and reducing the level of malondialdehyde(MDA).The differ-ent effects of each fraction on the expression of the oxidative stress factor indicated that each fraction has its unique mechanism against oxidative stress.In conclusion,this study provides a scientific ba-sis for the development and utilization of buckwheat hull as a natural antioxidant.
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