Effects of Sulfur Addition on Bacterial Community Diversity in Root Zone and Plant Growth and Development of Blueberry
Blueberry has a high requirement for soil pH and is suitable for growing in acid soil,and sulfur is used to adjust soil pH in production.Starting from the diversity differences of soil bacterial communities,the effects of soil pH on the growth and development of blueberry plants were explored,which provides a theoretical basis for the scientific management of blueberry soil pH."Bluecrop"cultivated in pot was used as test material,the effects of adding sulfur to regulate soil pH on the di-versity of rhizosphere and non rhizosphere bacterial communities,physiological functions of roots and leaves,and plant growth and development of blueberry were investigated.The results showed that after sulfur treatment,the three most abundat bacteria of blueberry rhizosphere and non rhizo-sphere soil at phylum level were Proteobacteria,unidentified Bacteria and Actinobacteria.The rela-tive abundance of Actinobacteria increased significantly after sulfur treatment,while the bacterial di-versity and community richness of rhizosphere and non rhizosphere soil of blueberry decreased sig-nificantly.The number of OTUs in control is more and in sulfur treatment is less,which is 1 556 and 160,respectively.Sulfur treatment significantly reduced the proline content and malondialdehyde content of blueberry leaves,which decreased by 25.91%and 14.58%compared with control,respec-tively.The chlorophyll content,root activity and total respiration rate increased significantly after sul-fur addition and the contribution rate of triglyceride cycle pathway in the basic respiratory biochemi-cal pathway was increased significantly.In the short term,sulfur treatment significantly reduced growth indicators such as plant height and leaf area of blueberry plants,but had no significant effect on growth indicators such as shoot dry weight,shoot fresh weight,root dry weight,root fresh weight,stem diameter and leaf number.The treatment of sulfur addition affected the growth and development of blueberry plants by reducing soil pH,changing soil microbial community structure,and affecting plant physiological functions.
blueberryrhizosphere bacterial diversityhigh throughput sequencingroot function